Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells

被引：17

作者：

Chiang, Yi-Fen ^{[1
]}

Tsai, Chih-Hung ^{[2
]}

Chen, Hsin-Yuan ^{[1
,3
]}

Wang, Kai-Lee ^{[4
]}

Chang, Hsin-Yi ^{[5
]}

Huang, Yun-Ju ^{[1
]}

Hong, Yong-Han ^{[3
]}

Ali, Mohamed ^{[6
]}

Shieh, Tzong-Ming ^{[7
]}

Huang, Tsui-Chin ^{[8
]}

Lin, Ching-, I ^{[9
]}

Hsia, Shih-Min ^{[1
,5
,10
,11
]}

机构：

[1] Taipei Med Univ, Coll Nutr, Sch Nutr & Hlth Sci, Taipei 11031, Taiwan

[2] Yu Kang Anim Hosp, New Taipei 220, Taiwan

[3] I Shou Univ, Dept Nutr, Kaohsiung 84001, Taiwan

[4] Ching Kuo Inst Management & Hlth, Dept Nursing, Keelung 20301, Taiwan

[5] Taipei Med Univ, Grad Inst Metab & Obes Sci, Taipei 11031, Taiwan

[6] Ain Shams Univ, Fac Pharm, Clin Pharm Dept, Cairo 11566, Egypt

[7] China Med Univ, Coll Dent, Sch Dent, Taichung 40402, Taiwan

[8] Taipei Med Univ, Coll Med Sci & Technol, Grad Inst Canc Biol & Drug Discovery, Taipei 11031, Taiwan

[9] Kainan Univ, Dept Nutr & Hlth Sci, Taoyuan 338, Taiwan

[10] Taipei Med Univ, Sch Food & Safety, Taipei 11031, Taiwan

[11] Taipei Med Univ Hosp, Nutr Res Ctr, Taipei 11031, Taiwan

来源：

MARINE DRUGS | 2021年 / 19卷 / 06期

关键词：

fucoxanthin; oxidative stress; calcification; heart valve interstitial cell; VASCULAR CALCIFICATION; OXIDATIVE STRESS; STENOSIS; EXPRESSION; INJURY; DOGS;

D O I：

10.3390/md19060307

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Cardiovascular diseases such as atherosclerosis and aortic valve sclerosis involve inflammatory reactions triggered by various stimuli, causing increased oxidative stress. This increased oxidative stress causes damage to the heart cells, with subsequent cell apoptosis or calcification. Currently, heart valve damage or heart valve diseases are treated by drugs or surgery. Natural antioxidant products are being investigated in related research, such as fucoxanthin (Fx), which is a marine carotenoid extracted from seaweed, with strong antioxidant, anti-inflammatory, and anti-tumor properties. This study aimed to explore the protective effect of Fx on heart valves under high oxidative stress, as well as the underlying mechanism of action. Rat heart valve interstitial cells under H2O2-induced oxidative stress were treated with Fx. Fx improved cell survival and reduced oxidative stress-induced DNA damage, which was assessed by cell viability analysis and staining with propidium iodide. Alizarin Red-S analysis indicated that Fx has a protective effect against calcification. Furthermore, Western blotting revealed that Fx abrogates oxidative stress-induced apoptosis via reducing the expression of apoptosis-related proteins as well as modulate Akt/ERK-related protein expression. Notably, in vivo experiments using 26 dogs treated with 60 mg/kg of Fx in combination with medical treatment for 0.5 to 2 years showed significant recovery in their echocardiographic parameters. Collectively, these in vitro and in vivo results highlight the potential of Fx to protect heart valve cells from high oxidative stress-induced damage.

引用

页数：13

共 43 条

[21] Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification [J].

Lin, Cui ;

Zhu, Dongxing ;

Markby, Greg ;

Corcoran, Brendan M. ;

Farquharson, Colin ;

Macrae, Vicky E. .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2017, (129)

[22] Protective Effect of Fucoxanthin Isolated from Laminaria japonica against Visible Light-Induced Retinal Damage Both in Vitro and in Vivo [J].

Liu, Yixiang ;

Liu, Meng ;

Zhang, Xichun ;

Chen, Qingchou ;

Chen, Haixiu ;

Sun, Lechang ;

Liu, Guangming .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (02) :416-424

[23] Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification [J].

Lu, Tzong-Shi ;

Lim, Kenneth ;

Molostvov, Guerman ;

Yang, Yun-Chun ;

Yiao, Szu-Yu ;

Zehnder, Daniel ;

Hsiao, Li-Li .

CARDIOVASCULAR RESEARCH, 2012, 96 (03) :524-532

[24] Endothelial-to-Mesenchymal Transition in Calcific Aortic Valve Disease [J].

Ma, Xiaochun ;

Zhao, Diming ;

Yuan, Peidong ;

Li, Finzhang ;

Yun, Van ;

Cui, Yuqi ;

Zhang, Tao ;

Ma, Jiwei ;

Sun, Liangong ;

Ma, Huibo ;

Zhang, Yuman ;

Zhang, Haizhou ;

Zhang, Wenlong ;

Huang, Junjie ;

Zou, Chengwei ;

Wang, Zhengjun .

ACTA CARDIOLOGICA SINICA, 2020, 36 (03) :183-194

[25] Maternal Supplementation of Inositols, Fucoxanthin, and Hydroxytyrosol in Pregnant Murine Models of Hypertension [J].

Menichini, Daniela ;

Alrais, Mesk ;

Liu, Chen ;

Xia, Yang ;

Blackwell, Sean C. ;

Facchinetti, Fabio ;

Sibai, Baha M. ;

Longo, Monica .

AMERICAN JOURNAL OF HYPERTENSION, 2020, 33 (07) :652-659

[26] The emerging role of nuclear architecture in DNA repair and genome maintenance [J].

Misteli, Tom ;

Soutoglou, Evi .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (04) :243-254

[27] Valvular osteoclasts in calcification and aortic valve stenosis severity [J].

Nagy, Edit ;

Eriksson, Per ;

Yousry, Mohamed ;

Caidahl, Kenneth ;

Ingelsson, Erik ;

Hansson, Goran K. ;

Franco-Cereceda, Anders ;

Back, Magnus .

INTERNATIONAL JOURNAL OF CARDIOLOGY, 2013, 168 (03) :2264-2271

[28]

Nakayama H, 2001, J VET INTERN MED, V15, P217, DOI 10.1892/0891-6640(2001)015<0217:COCEAA>2.3.CO

[29]

[30] OxLDL-derived lysophosphatidic acid promotes the progression of aortic valve stenosis through a LPAR1-RhoA-NF-κB pathway [J].

Nsaibia, Mohamed Jalloul ;

Boulanger, Marie-Chloe ;

Bouchareb, Rihab ;

Mkannez, Ghada ;

Le Quang, Khai ;

Hadji, Fayez ;

Argaud, Deborah ;

Dahou, Abdellaziz ;

Bosse, Yohan ;

Koschinsky, Marlys L. ;

Pibarot, Philippe ;

Arsenault, Benoit J. ;

Marette, Andre ;

Mathieu, Patrick .

CARDIOVASCULAR RESEARCH, 2017, 113 (11) :1351-1363

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