Systemic and vascular inflammation in an in-vitro model of central obesity

被引:23
作者
Ahluwalia, Arti [1 ]
Misto, Alessandra [2 ]
Vozzi, Federico [2 ]
Magliaro, Chiara [1 ]
Mattei, Giorgio [1 ]
Marescotti, Maria Cristina [3 ]
Avogaro, Angelo [3 ]
Iori, Elisabetta [3 ]
机构
[1] Univ Pisa, Res Ctr E Piaggio, Pisa, Italy
[2] Italian Natl Council Res, Inst Clin Physiol, Pisa, Italy
[3] Univ Padua, Dept Med, Padua, Italy
关键词
FREE FATTY-ACIDS; ADIPOSE-TISSUE; METABOLIC SYNDROME; ENDOTHELIAL DYSFUNCTION; INSULIN-RESISTANCE; LACTATE PRODUCTION; LIPID-METABOLISM; GLUCOSE; CELL; DIFFERENTIATION;
D O I
10.1371/journal.pone.0192824
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Metabolic disorders due to over-nutrition are a major global health problem, often associated with obesity and related morbidities. Obesity is peculiar to humans, as it is associated with lifestyle and diet, and so difficult to reproduce in animal models. Here we describe a model of human central adiposity based on a 3-tissue system consisting of a series of interconnected fluidic modules. Given the causal link between obesity and systemic inflammation, we focused primarily on pro-inflammatory markers, examining the similarities and differences between the 3-tissue model and evidence from human studies in the literature. When challenged with high levels of adiposity, the in-vitro system manifests cardiovascular stress through expression of E-selectin and von Willebrand factor as well as systemic inflammation (expressing IL-6 and MCP-1) as observed in humans. Interestingly, most of the responses are dependent on the synergic interaction between adiposity and the presence of multiple tissue types. The set-up has the potential to reduce animal experiments in obesity research and may help unravel specific cellular mechanisms which underlie tissue response to nutritional overload.
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页数:15
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