Determination of dexamethasone and two excipients (creatinine and propylparaben) in injections by using UV-spectroscopy and multivariate calibrations

被引：23

作者：

Collado, MS ^{[1
]}

Robles, JC ^{[1
]}

De Zan, M ^{[1
]}

Cámara, MS ^{[1
]}

Mantovani, VE ^{[1
]}

Goicoechea, HC ^{[1
]}

机构：

[1] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Lab Control Calidad Medicamentos, Catedra Quim Analit 1, RA-3000 Paraje El Pozo, Santa Fe, Argentina

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2001年 / 229卷 / 1-2期

关键词：

dexamethasone; creatinine; propylparaben; multivariate calibration; spectrophotometry;

D O I：

10.1016/S0378-5173(01)00866-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The use of multivariate spectrophotometric calibration for the simultaneous determination of dexamethasone and two typical excipients (creatinine and propylparaben) in injections is presented. The resolution of the three-component mixture in a matrix of excipients has been accomplished by using partial least-squares (PLS-1). Notwithstanding the elevated degree of spectral overlap, they have been rapidly and simultaneously determined with high accuracy and precision (comparable to the HPLC pharmacopeial method), with no interference, and without resorting to extraction procedures using non-aqueous solvents. A simple and fast method for wavelength selection in the calibration step is used, based on the minimisation of the predicted error sum of squares (PRESS) calculated as a function of a moving spectral window. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：205 / 211

页数：7

共 21 条

[1]

Chen X., 1991, PLASMA CHEM PLASMA P, V11, P103

[2] Simultaneous spectrophotometric-multivariate calibration determination of several components of ophthalmic solutions: phenylephrine, chloramphenicol, antipyrine, methylparaben and thimerosal [J].

Collado, MS ;

Mantovani, VE ;

Goicoechea, HC ;

Olivieri, AC .

TALANTA, 2000, 52 (05) :909-920

[3] THIN-LAYER CHROMATOGRAPHIC METHOD FOR RAPID IDENTIFICATION AND QUANTIFICATION OF CORTICOSTEROID SODIUM PHOSPHATES IN PHARMACEUTICAL PREPARATIONS [J].

DAS, B ;

CHATTERJEE, SK ;

DAS, SK .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1986, 9 (16) :3461-3467

[4] QUANTITATIVE DEXAMETHASONE AND DEXAMETHASONE SODIUM-PHOSPHATE DETERMINATIONS IN PHARMACEUTICAL DOSAGE FORMS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

DASGUPTA, V .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1979, 68 (07) :926-928

[5]

Datta Kajal, 1993, Journal of Planar Chromatography - Modern TLC, V6, P204

[6] ANALYSIS OF DEXAMETHASONE SODIUM-PHOSPHATE FORMULATIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

GAGNE, D ;

LODGE, BA .

JOURNAL OF CHROMATOGRAPHY, 1980, 193 (01) :160-162

[7] Determination of theophylline in blood serum by UV spectrophotometry and partial least-squares (PLS-1) calibration [J].

Goicoechea, HC ;

Olivieri, AC ;

de la Peña, AM .

ANALYTICA CHIMICA ACTA, 1999, 384 (01) :95-103

[8] MULTIVAR. A program for multivariate calibration incorporating net analyte signal calculations [J].

Goicoechea, HC ;

Olivieri, AC .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2000, 19 (10) :599-605

[9] Enhanced synchronous spectrofluorometric determination of tetracycline in blood serum by chemometric analysis. Comparison of partial least-squares and hybrid linear analysis calibrations [J].

Goicoechea, HC ;

Olivieri, AC .

ANALYTICAL CHEMISTRY, 1999, 71 (19) :4361-4368

[10] Determination of bromhexine in cough-cold syrups by absorption spectrophotometry and multivariate calibration using partial least-squares and hybrid linear analyses. Application of a novel method of wavelength selection [J].

Goicoechea, HC ;

Olivieri, AC .

TALANTA, 1999, 49 (04) :793-800

← 1 2 3 →