Spatially addressable arrays of molecules embedded in or anchored to supported lipid bilayers are important for on-chip screening and binding assays; however, methods to sort or accumulate components in a fluid membrane on demand are still limited. Here we apply in-plane surface acoustic shear waves (SAWs) to laterally accumulate double-stranded DNA segments electrostatically bound to a cationic supported lipid bilayer. The fluorescently labeled DNA segments are found to segregate into stripe patterns with a spatial frequency corresponding to the periodicity of the standing SAW wave (similar to 10 mu m). The DNA molecules are accumulated 10-fold in the regions of SAW antinodes. The superposition of two orthogonal sets of SAW sources creates checkerboard like arrays of DNA demonstrating the potential to generate arrayed fields dynamically. The pattern relaxation time of 0.58 s, which is independent of the segment length, indicates a sorting and relaxation mechanism dominated by lipid diffusion rather than DNA self-diffusion.
机构:
Cooperat Res Ctr Biomat CIC BiomaGUNE, Biosurfaces Unit, San Sebastian, SpainCooperat Res Ctr Biomat CIC BiomaGUNE, Biosurfaces Unit, San Sebastian, Spain
Carton, Ixaskun
Malinina, Lucy
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Cooperat Res Ctr Biosci CIC BioGUNE, Struct Biol Unit, Derio, SpainCooperat Res Ctr Biomat CIC BiomaGUNE, Biosurfaces Unit, San Sebastian, Spain
Malinina, Lucy
Richter, Ralf P.
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Cooperat Res Ctr Biomat CIC BiomaGUNE, Biosurfaces Unit, San Sebastian, Spain
Max Planck Inst Met Res, D-70569 Stuttgart, GermanyCooperat Res Ctr Biomat CIC BiomaGUNE, Biosurfaces Unit, San Sebastian, Spain
机构:
Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Goksu, Emel I.
Hoopes, Matthew I.
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Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Hoopes, Matthew I.
Nellis, Barbara A.
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Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Lawrence Livermore Natl Lab, Chem Mat Earth & Life Sci Directorate, Livermore, CA 94550 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Nellis, Barbara A.
Xing, Chenyue
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Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Xing, Chenyue
Faller, Roland
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Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Faller, Roland
Frank, Curtis W.
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Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Frank, Curtis W.
Risbud, Subhash H.
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Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Risbud, Subhash H.
Satcher, Joe H., Jr.
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Lawrence Livermore Natl Lab, Chem Mat Earth & Life Sci Directorate, Livermore, CA 94550 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
Satcher, Joe H., Jr.
Longo, Marjorie L.
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Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USAUniv Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA
机构:
Lund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Lund Univ, NanoLund, SE-22100 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Dabkowska, Aleksandra P.
Piret, Gaelle
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Lund Univ, NanoLund, SE-22100 Lund, Sweden
Lund Univ, Div Solid State Phys, SE-22100 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Piret, Gaelle
Niman, Cassandra S.
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Lund Univ, NanoLund, SE-22100 Lund, Sweden
Lund Univ, Div Solid State Phys, SE-22100 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Niman, Cassandra S.
Lard, Mercy
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Lund Univ, NanoLund, SE-22100 Lund, Sweden
Lund Univ, Div Solid State Phys, SE-22100 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Lard, Mercy
Linke, Heiner
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Lund Univ, NanoLund, SE-22100 Lund, Sweden
Lund Univ, Div Solid State Phys, SE-22100 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Linke, Heiner
Nylander, Tommy
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Lund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Lund Univ, NanoLund, SE-22100 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden
Nylander, Tommy
Prinz, Christelle N.
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Lund Univ, NanoLund, SE-22100 Lund, Sweden
Lund Univ, Div Solid State Phys, SE-22100 Lund, Sweden
Lund Univ, Neuronano Res Ctr, SE-22184 Lund, SwedenLund Univ, Div Phys Chem, SE-22100 Lund, Sweden