Identification and Characterization of a Novel Umbra-like Virus, Strawberry Virus A, Infecting Strawberry Plants

被引:13
作者
Koloniuk, Igor [1 ]
Pribylova, Jaroslava [1 ]
Cmejla, Radek [2 ]
Valentova, Lucie [2 ]
Franova, Jana [1 ]
机构
[1] Czech Acad Sci, Inst Plant Mol Biol, Dept Plant Virol, Biol Ctr, Branisovska 31, Ceske Budejovice 37005, Czech Republic
[2] Res & Breeding Inst Pomol Holovousy Ltd, Holovousy 129, Horice 50801, Czech Republic
来源
PLANTS-BASEL | 2022年 / 11卷 / 05期
关键词
novel virus; strawberry; high-throughput sequencing; UMBRAVIRUSES; COMBINATION;
D O I
10.3390/plants11050643
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A novel RNA virus infecting strawberry plants was discovered using high-throughput sequencing. The analyzed plant was simultaneously infected with three different genetic variants of the virus, provisionally named strawberry virus A (StrVA). Although StrVA is phylogenetically clustered with several recently discovered, unclassified plant viruses, it has a smaller genome and several unique features in its genomic organization. A specific and sensitive qPCR system for the detection of identified StrVA genetic variants was designed. A survey conducted in the Czech Republic revealed that StrVA was present in 28.3% of strawberry samples (n = 651) from various origins (plantations, gardens, and propagation material). Sequencing of 48 randomly selected StrVA-positive strawberry samples showed that two or all three StrVA genetic variants were present in 62.5% of the samples in various proportions. StrVA was found in mixed infections with other viruses (strawberry mild yellow edge virus, strawberry crinkle virus, strawberry mottle virus, strawberry polerovirus 1, or strawberry virus 1) in 57.1% of the samples, which complicated the estimation of its biological relevance and impact on the health status of the plants.
引用
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页数:12
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