Characterization of dihydroartemisinin-resistant colon carcinoma HCT116/R cell line
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作者:
Lu, Jin-Jian
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Zhejiang Chinese Med Univ, Coll Life Sci, Hangzhou 310053, Zhejiang, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Lu, Jin-Jian
[1
,2
]
Chen, Si-Meng
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Chen, Si-Meng
[1
]
Ding, Jian
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Ding, Jian
[1
]
Meng, Ling-Hua
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Meng, Ling-Hua
[1
]
机构:
[1] Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
[2] Zhejiang Chinese Med Univ, Coll Life Sci, Hangzhou 310053, Zhejiang, Peoples R China
Dihydroartemisinin (DHA) is an important artemisinin derivative and presents profound anti-tumor potential. A DHA-resistant cell line named HCT116/R derived from colon carcinoma cell line HCT116 was established in our previous study. Herein, we found that HCT116/R cells were much more resistant to DHA- or artesunate-induced proliferation inhibition and more tolerant to DHA-induced cell cycle arrest and apoptosis compared with those of the parent HCT116 cells. The protein levels of P-glycoprotein and MDR-associated protein 1 and the accumulation of doxorubicin in cells were similar in both cell lines. Moreover, HCT116/R cells were still sensitive to camptothecin- and doxorubicin-induced cell growth inhibition. To further explore the characterization of HCT116/R cell line, a proteomic study employing two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was performed. Eight different expressed proteins between the two cell lines were identified including some heat shock proteins, annexins, etc. This study not only indicates that exposure to DHA may not induce a tumor multi-drug-resistant phenotype but also affords new clues for the further investigation of the anti-cancer mechanisms of DHA and other artemisinin derivatives.
机构:
Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Lu, Jin-Jian
Chen, Si-Meng
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Chen, Si-Meng
Zhang, Xiao-Wei
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Zhang, Xiao-Wei
Ding, Jian
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
Ding, Jian
Meng, Ling-Hua
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Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R ChinaChinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
机构:
King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Alqarni, Aisha
Elnakady, Yasser A.
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King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Elnakady, Yasser A.
Alsadhan, Lamya
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King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Alsadhan, Lamya
Abbas, Muhammad
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Tube Pharmaceut GmbH Biotech Competence Ctr BCC, Leberstr 20, A-1110 Vienna, AustriaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Abbas, Muhammad
Richter, Wolfgang
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Tube Pharmaceut GmbH Biotech Competence Ctr BCC, Leberstr 20, A-1110 Vienna, AustriaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Richter, Wolfgang
Aldahmash, Badr A.
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King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Aldahmash, Badr A.
Almansour, Mansour I.
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King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Almansour, Mansour I.
Almutairi, Layali M.
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King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
Almutairi, Layali M.
Rady, Ahmed
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King Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi ArabiaKing Saud Univ, Coll Sci, Dept Zool, BO Box 2455, Riyadh 11415, Saudi Arabia
机构:
Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Student Res Comm, Tehran, IranShahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Student Res Comm, Tehran, Iran
Mirzarazi, Mahsa
Bashiri, Soroor
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Shahid Beheshti Univ Med Sci, Sch Med, Dept Pathol, Tehran, IranShahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Student Res Comm, Tehran, Iran
Bashiri, Soroor
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Hashemi, Ali
Vahidi, Mahmoud
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Aja Univ Med Sci, Sch Allied Med Sci, Dept Med Lab Sci, Tehran, IranShahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Student Res Comm, Tehran, Iran
Vahidi, Mahmoud
Kazemi, Bahram
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Shahid Beheshti Univ Med Sci, Cellular & Mol Biol Res Ctr, Tehran, IranShahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Student Res Comm, Tehran, Iran
机构:Shahid Beheshti University of Medical Sciences,Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine
Mahsa Mirzarazi
Soroor Bashiri
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机构:Shahid Beheshti University of Medical Sciences,Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine
Soroor Bashiri
Ali Hashemi
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机构:Shahid Beheshti University of Medical Sciences,Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine
Ali Hashemi
Mahmoud Vahidi
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机构:Shahid Beheshti University of Medical Sciences,Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine
Mahmoud Vahidi
Bahram Kazemi
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机构:Shahid Beheshti University of Medical Sciences,Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine
Bahram Kazemi
Mojgan Bandehpour
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机构:Shahid Beheshti University of Medical Sciences,Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine