Adaptation and Diagnostic Potential of a Commercial Cat Interferon Gamma Release Assay for the Detection of Mycobacterium bovis Infection in African Lions (Panthera leo)

被引：8

作者：

Gumbo, Rachiel ^{[1
,2
,3
]}

Sylvester, Tashnica T. ^{[1
,2
,3
]}

Goosen, Wynand J. ^{[1
,2
,3
]}

Buss, Peter E. ^{[4
]}

De Klerk-Lorist, Lin-Mari ^{[5
]}

van Schalkwyk, O. Louis ^{[5
,6
,7
]}

McCall, Alicia ^{[8
]}

Warren, Robin M. ^{[1
,2
,3
]}

van Helden, Paul D. ^{[1
,2
,3
]}

Miller, Michele A. ^{[1
,2
,3
]}

Kerr, Tanya J. ^{[1
,2
,3
]}

机构：

[1] Stellenbosch Univ, Fac Med & Hlth Sci, DSI NRF Ctr Excellence Biomed TB Res, ZA-7505 Tygerberg, South Africa

[2] Stellenbosch Univ, Fac Med & Hlth Sci, SAMRC Ctr TB Res, ZA-7505 Tygerberg, South Africa

[3] Stellenbosch Univ, Fac Med & Hlth Sci, Div Mol Biol & Human Genet, ZA-7505 Tygerberg, South Africa

[4] Kruger Natl Pk, Vet Wildlife Serv, South African Natl Pk, ZA-1350 Skukuza, South Africa

[5] Dept Agr Land Reform & Rural Dev, Skukuza State Vet Off, ZA-1350 Skukuza, South Africa

[6] Univ Pretoria, Fac Vet Sci, Dept Vet Trop Dis, ZA-0110 Pretoria, South Africa

[7] Max Planck Inst Anim Behav, Dept Migrat, D-78315 Radolfzell am Bodensee, Germany

[8] Kwazulu Natal Dept Agr & Rural Dev, Hluhluwe State Vet Off, ZA-3960 Hluhluwe, South Africa

来源：

PATHOGENS | 2022年 / 11卷 / 07期

基金：

新加坡国家研究基金会; 英国医学研究理事会;

关键词：

African lion; bovine tuberculosis; cytokine ELISA; IFN-gamma; interferon-gamma release assay; Mycobacterium bovis; Panthera leo; TUBERCULOSIS; CONSERVATION;

D O I：

10.3390/pathogens11070765

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacterium bovis (M. bovis) infection in wildlife, including lions (Panthera leo), has implications for individual and population health. Tools for the detection of infected lions are needed for diagnosis and disease surveillance. This study aimed to evaluate the Mabtech Cat interferon gamma (IFN-gamma) ELISABasic kit for detection of native lion IFN-gamma in whole blood samples stimulated using the QuantiFERON (R) TB Gold Plus (QFT) platform as a potential diagnostic assay. The ELISA was able to detect lion IFN-gamma in mitogen-stimulated samples, with good parallelism, linearity, and a working range of 15.6-500 pg/mL. Minimal matrix interference was observed in the recovery of domestic cat rIFN-gamma in lion plasma. Both intra- and inter-assay reproducibility had a coefficient of variation less than 10%, while the limit of detection and quantification were 7.8 pg/mL and 31.2 pg/mL, respectively. The diagnostic performance of the QFT Mabtech Cat interferon gamma release assay (IGRA) was determined using mycobacterial antigen-stimulated samples from M. bovis culture-confirmed infected (n = 8) and uninfected (n = 4) lions. A lion-specific cut-off value (33 pg/mL) was calculated, and the sensitivity and specificity were determined to be 87.5% and 100%, respectively. Although additional samples should be tested, the QFT Mabtech Cat IGRA could identify M. bovis-infected African lions.

引用

页数：12

共 40 条

[1] Multi-host pathogens and carnivore management in southern Africa [J].

Alexander, K. A. ;

McNutt, J. W. ;

Briggs, M. B. ;

Standers, P. E. ;

Funston, P. ;

Hemson, G. ;

Keet, D. ;

van Vuuren, M. .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2010, 33 (03) :249-265

[2] A practical guide to immunoassay method validation [J].

Andreasson, Ulf ;

Perret-Liaudet, Armand ;

van Doorn, Linda J. C. van Waalwijk ;

Blennow, Kaj ;

Chiasserini, Davide ;

Engelborghs, Sebastiaan ;

Fladby, Tormod ;

Genc, Sermin ;

Kruse, Niels ;

Kuipenj, H. Bea ;

Kulic, Luka ;

Lewczuk, Piotr ;

Mollenhauer, Brit ;

Mroczko, Barbara ;

Pametti, Lucille ;

Vanmechelen, Eugeen ;

Verbeek, Marcel M. ;

Winblad, Bengt ;

Zetterberg, Henrik ;

Koel-Simmelink, Marleen ;

Teunissen, Charlotte E. .

FRONTIERS IN NEUROLOGY, 2015, 6

[3]

[Anonymous], 2016, IUCN RED LIST THREAT

[4]

Armbruster David A, 2008, Clin Biochem Rev, V29 Suppl 1, pS49

[5] Computing the noncentral-F distribution and the power of the F-test with guaranteed accuracy [J].

Baharev, Ali ;

Schichl, Hermann ;

Rev, Endre .

COMPUTATIONAL STATISTICS, 2017, 32 (02) :763-779

[6]

Banoo S, 2006, NAT REV MICROBIOL, pS21, DOI [10.1038/nrmico1523, 10.1038/nrmicro1523]

[7] Review of Diagnostic Tests for Detection of Mycobacterium bovis Infection in South African Wildlife [J].

Bernitz, Netanya ;

Kerr, Tanya J. ;

Goosen, Wynand J. ;

Chileshe, Josephine ;

Higgitt, Roxanne L. ;

Roos, Eduard O. ;

Meiring, Christina ;

Gumbo, Rachiel ;

de Waal, Candice ;

Clarke, Charlene ;

Smith, Katrin ;

Goldswain, Samantha ;

Sylvester, Taschnica T. ;

Kleynhans, Leanie ;

Dippenaar, Anzaan ;

Buss, Peter E. ;

Cooper, David, V ;

Lyashchenko, Konstantin P. ;

Warren, Robin M. ;

van Helden, Paul D. ;

Parsons, Sven D. C. ;

Miller, Michele A. .

FRONTIERS IN VETERINARY SCIENCE, 2021, 8

[8] Parallel testing increases detection of Mycobacterium bovis-infected African buffaloes (Syncerus caffer) [J].

Bernitz, Netanya ;

Goosen, Wynand J. ;

Clarke, Charlene ;

Kerr, Tanya J. ;

Higgitt, Roxanne ;

Roos, Eduard O. ;

Cooper, David, V ;

Warren, Robin M. ;

van Helden, Paul D. ;

Parsons, Sven D. C. ;

Miller, Michele A. .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2018, 204 :40-43

[9] An interferon-gamma release assay for the diagnosis of the Mycobacterium bovis infection in white rhinoceros (Ceratotherium simum) [J].

Chileshe, Josephine ;

Roos, Eduard O. ;

Goosen, Wynand J. ;

Buss, Peter ;

Hausler, Guy ;

Rossouw, Leana ;

Manemela, Tebogo ;

van Helden, Paul ;

Warren, Robin ;

Parsons, Sven D. C. ;

Miller, Michele .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2019, 217

[10]

Cox K.L., 2012, ASSAY GUID MAN

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