Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

被引：28

作者：

Nishikawa, Shimpei ^{[1
,2
]}

Konno, Masamitsu ^{[1
]}

Hamabe, Atsushi ^{[1
,2
]}

Hasegawa, Shinichiro ^{[1
,2
]}

Kano, Yoshihiro ^{[1
,2
]}

Fukusumi, Takahito ^{[3
]}

Satoh, Taroh ^{[1
]}

Takiguchi, Shuji ^{[2
]}

Mori, Masaki ^{[2
]}

Doki, Yuichiro ^{[2
]}

Ishii, Hideshi ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Frontier Sci Canc & Chemotherapy, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Med, Dept Gastroenterol Surg, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Grad Sch Med, Dept Otorhinolaryngol Head & Neck Surg, Suita, Osaka 5650871, Japan

来源：

ONCOLOGY LETTERS | 2015年 / 9卷 / 05期

关键词：

gastric cancer; cancer stem cell; tumor-initiating cell; chemotherapy; CD44; CD26; ACUTE MYELOID-LEUKEMIA; INITIATING CELLS; IDENTIFICATION; CHEMOTHERAPY;

D O I：

10.3892/ol.2015.3063

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti-cell surface marker antibody-based array platform, a flow cytometry-based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26(+)CD44(+) cells (6/6 mice formed tumors), moderately tumorigenic CD26(+)CD44(-) cells (5/6 mice formed tumors), and weakly or non-tumorigenic CD26(-)CD44(-) cells (2/6 mice formed tumors). Furthermore, exposure to 5-fluorouracil significantly increased the proportion of CD26(+) cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.

引用

页码：2361 / 2367

页数：7