Spatial distribution of two-photon-excited fluorescence in scattering media

被引：56

作者：

Ying, YP ^{[1
]}

Liu, F

Alfano, RR

机构：

[1] CUNY City Coll, Dept Phys, New York State Ctr Adv Technol Ultrafast Photon M, Inst Ultrafast Spect & Lasers, New York, NY 10031 USA

[2] CUNY, Grad Sch, New York, NY 10031 USA

来源：

APPLIED OPTICS | 1999年 / 38卷 / 01期

关键词：

D O I：

10.1364/AO.38.000224

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Spatial distribution of two-photon-excited fluorescence (TPF) for dye beneath the surface of a highly scattering medium was investigated dth picosecond laser pulses at 1064 nm. The active scattering media consisted of a suspension of polystyrene particles in a solution of Rhodamine 590 Tetrafluoroborate dye. With the increase of scattering strength of the medium, the location of the maximum TPF intensity was found to move closer to the surface away from the focal region; the intensity of TPF was not confined to the focal region as in the case of nonscattering medium but was more evenly distributed. The spatial resolution of nonlinear optical microscopy for probing a scattering medium is degraded. Taking into account the scattering of the medium qualitatively explains the observed TPF spatial distribution. (C) 1999 Optical Society of America.

引用

页码：224 / 229

页数：6

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