Development of a p72 trimer-based colloidal gold strip for detection of antibodies against African swine fever virus

被引：52

作者：

Geng, Rui ^{[1
,2
]}

Sun, Yaning ^{[2
]}

Li, Rui ^{[2
]}

Yang, Jifei ^{[2
]}

Ma, Hongfang ^{[2
]}

Qiao, Zixuan ^{[3
]}

Lu, Qingxia ^{[2
]}

Qiao, Songlin ^{[2
]}

Zhang, Gaiping ^{[1
,2
]}

机构：

[1] Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Henan, Peoples R China

[2] Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China

[3] Henan Expt High Sch, Zhengzhou 450002, Henan, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2022年 / 106卷 / 07期

关键词：

Antibody detection; ASFV; Colloidal gold immunochromatographic strip; P72; trimers; MAJOR CAPSID PROTEIN; EXPRESSION;

D O I：

10.1007/s00253-022-11851-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

African swine fever virus (ASFV) causes a highly contagious and often lethal swine viral disease, and leads to tremendous economic losses to the swine industry. Unfortunately, there are no vaccines and effective antiviral agents available to prevent and control ASFV outbreaks. Therefore, it is necessary to develop simple and rapid strategies to monitor ASFV-infected pigs to restrain its spread. In the current study, ASFV capsid protein p72 was expressed along with its chaperone pB602L to form trimers in human embryonic kidney 293 (HEK293) cells. The p72 trimers were subsequently labeled with colloidal gold to develop a immunochromatographic strip. The strip showed high specificity to ASFV-positive serum and no cross-reactivity to other swine virus positive sera. Importantly, the strip showed a higher sensitivity of detecting ASFV antibodies in both positive standard serum and clinical serum samples than a commercial enzyme-linked immunosorbent assay (ELISA) kit. Taken together, these results demonstrate the strip as a reliable diagnostic tool against ASFV infection, which will be appropriate for application in prevention and control of ASFV.

引用

页码：2703 / 2714

页数：12

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