Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags

被引：16

作者：

Wardrop, J ^{[1
]}

Fuller, J ^{[1
]}

Powell, W ^{[1
]}

Machray, GC ^{[1
]}

机构：

[1] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland

来源：

THEORETICAL AND APPLIED GENETICS | 2004年 / 108卷 / 02期

关键词：

D O I：

10.1007/s00122-003-1434-6

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated.

引用

页码：343 / 348

页数：6

共 21 条

[1]

BOEHNKE M, 1991, AM J HUM GENET, V49, P1174

[2] NUTRITIONAL-REQUIREMENTS OF PROTOPLAST-DERIVED, HAPLOID TOBACCO CELLS GROWN AT LOW CELL DENSITIES IN LIQUID-MEDIUM [J].