Feeding effects of dsNPF interference in Ostrinia furnacalis

被引：11

作者：

Cui Hong-ying ^{[1
]}

Wang Yuan ^{[1
]}

Peng Xin ^{[1
]}

Wang Yi-tong ^{[1
]}

Zhao Zhang-wu ^{[1
]}

机构：

[1] China Agr Univ, Coll Plant Protect, Dept Entomol, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China

来源：

JOURNAL OF INTEGRATIVE AGRICULTURE | 2020年 / 19卷 / 06期

基金：

中国国家自然科学基金;

关键词：

neuropeptide; NPF; NPFR; feeding regulation; Asian corn borer; NEUROPEPTIDE-Y-LIKE; NERVOUS-SYSTEM; DESERT LOCUST; INSULIN-LIKE; DROSOPHILA; FLUORESCENT; FOOD; RNA;

D O I：

10.1016/S2095-3119(19)62788-7

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The corn borer is a world-wide agricultural pest. In this study, a high-efficiency RNAi method was explored to knock down the neuropeptide F (NPF) to determine if NPF regulates larval feeding in the Asian corn borer, Ostrinia fumacalis. Results showed that the expression peaks of npf mRNA in both midgut and fat body are at 28 h of early 5th instar larvae. When NPF was knocked down either by feeding dsNPF-containing artificial diet or direct dsNPF injection into the 5th instar larvae, npf expression was effectively inhibited in the midgut. Larval feeding, body weight and development time were significantly impacted. In contrast, the expression of the npf receptors npfrl and npfr2 in fat body of 5th instar larvae was significantly increased by dsNPF injection. These results indicate that NPF can be effectively knocked down in O. furnacalis, and dsNPF by injection is an effective and fast way to silence npf expression. This study provides a critical basis for further exploration in mechanism of feeding regulation in O. furnacalis.

引用

页码：1475 / 1481

页数：7

共 42 条

[1]

Altstein M, 2010, ADV EXP MED BIOL, V692, P155

[2] 'Becoming a species by becoming a pest' or how two maize pests of the genus Ostrinia possibly evolved through parallel ecological speciation events [J].