Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy

被引：41

作者：

Prado, Enora ^{[1
,2
]}

Daugey, Nicolas ^{[1
]}

Plumet, Sebastien ^{[3
]}

Servant, Laurent ^{[1
]}

Lecomte, Sophie ^{[2
]}

机构：

[1] Univ Bordeaux 1, CNRS, Inst Mol Sci, F-33405 Talence, France

[2] Univ Bordeaux 1, CNRS, F-33607 Pessac, France

[3] DGA D4S MRIS, F-92250 Bagneux, France

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 26期

关键词：

SCATTERING; SILVER; DNA; SERS;

D O I：

10.1039/c1cc11925g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Surface-Enhanced Raman Spectroscopy (SERS) was performed to detect label-free RNA. We defined conditions which make it possible to probe the four bases of RNA, in single strands of polyadenosine (pA), polyuridine (pU), polycytosine (pC) and polyguanosine (pG). We therefore present below a quantitative analysis of mixtures of non-hybridized single strands, based on the deconvolution of the SERS mixture spectrum into the relative contributions of the SERS spectra of each constituent.

引用

页码：7425 / 7427

页数：3

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