Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary

被引：7

作者：

Doszpoly, Andor ^{[1
]}

Papp, Melitta ^{[2
]}

Deakne, Petra P. ^{[2
]}

Glavits, Robert ^{[2
]}

Ursu, Krisztina ^{[2
]}

Dan, Adam ^{[2
]}

机构：

[1] Hungarian Acad Sci, Vet Med Res Inst, Agr Res Ctr, H-1581 Budapest, Hungary

[2] Natl Food Chain Safety Off, Vet Diagnost Directorate, Budapest, Hungary

来源：

ARCHIVES OF VIROLOGY | 2015年 / 160卷 / 05期

基金：

匈牙利科学研究基金会;

关键词：

Alloherpesviridae; Cyprinid herpesvirus; Pelecus cultratus; Virus taxonomy; GENOME; SEQUENCE; KOI;

D O I：

10.1007/s00705-015-2348-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the early summer of 2014, mass mortality of sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus.

引用

页码：1279 / 1283

页数：5

共 26 条

[21]

Pellett P., 2011, Virus Taxonomy-Ninth Report of the International Committee on Taxonomy of Viruses, P99

[22]

Sano T., 1985, Fish and shellfish pathology. Proceedings of the European Association of Fish Pathologists, Plymouth, September 1983., P307

[23] First detection of a viral agent causing disease in farmed sturgeon in Russia [J].

Shchelkunov, Igor S. ;

Shchelkunova, Tatiana I. ;

Shchelkunov, Artyem I. ;

Kolbassova, Yulia P. ;

Didenko, Lubov V. ;

Bykovsky, Albert P. .

DISEASES OF AQUATIC ORGANISMS, 2009, 86 (03) :193-203

[24] The Staden sequence analysis package [J].

Staden, R .

MOLECULAR BIOTECHNOLOGY, 1996, 5 (03) :233-241

[25] Complete genome sequence and taxonomic position of anguillid herpesvirus 1 [J].

van Beurden, Steven J. ;

Bossers, Alex ;

Voorbergen-Laarman, Michal H. A. ;

Haenen, Olga L. M. ;

Peters, Sander ;

Abma-Henkens, Marleen H. C. ;

Peeters, Ben P. H. ;

Rottier, Peter J. M. ;

Engelsma, Marc Y. .

JOURNAL OF GENERAL VIROLOGY, 2010, 91 :880-887

[26] Phylogenetic relationships in the family Alloherpesviridae [J].

Waltzek, Thomas B. ;

Kelley, Garry O. ;

Alfaro, Michael E. ;

Kurobe, Tomofumi ;

Davison, Andrew J. ;

Hedrick, Ronald P. .

DISEASES OF AQUATIC ORGANISMS, 2009, 84 (03) :179-194

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