Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022

被引：57

作者：

Paran, Nir ^{[1
]}

Yahalom-Ronen, Yfat ^{[1
]}

Shifman, Ohad ^{[2
]}

Lazar, Shirley ^{[2
]}

Ben-Ami, Ronen ^{[3
,10
]}

Yakubovsky, Michal ^{[3
]}

Levy, Itzchak ^{[4
,10
]}

Wieder-Feinsod, Anat ^{[4
,10
]}

Amit, Sharon ^{[5
]}

Katzir, Michal ^{[6
,10
]}

Carmi-Oren, Noga ^{[7
]}

Levcovich, Ariela ^{[7
]}

Hershman-Sarafov, Mirit ^{[8
,11
]}

Paz, Alona ^{[8
,11
]}

Thomas, Rebecca ^{[9
]}

Tamir, Hadas ^{[1
]}

Cherry-Mimran, Lilach ^{[1
]}

Erez, Noam ^{[1
]}

Melamed, Sharon ^{[1
]}

Barlev-Gross, Moria ^{[1
]}

Karmi, Shay ^{[1
]}

Politi, Boaz ^{[1
]}

Achdout, Hagit ^{[1
]}

Weiss, Shay ^{[1
]}

Levy, Haim ^{[1
]}

Schuster, Ofir ^{[1
]}

Beth-Din, Adi ^{[2
]}

Israely, Tomer ^{[1
]}

机构：

[1] Israel Inst Biol Res, Dept Infect Dis, Ness Ziona, Israel

[2] Israel Inst Biol Res, Dept Biochem & Mol Genet, Ness Ziona, Israel

[3] Tel Aviv Sourasky Med Ctr, Infect Dis Dept, Tel Aviv, Israel

[4] Sheba Med Ctr, Infect Dis Unit, Ramat Gan, Israel

[5] Sheba Med Ctr, Clin Microbiol, Ramat Gan, Israel

[6] Meir Med Ctr, Infect Dis Unit, Kefar Sava, Israel

[7] Shamir Assaf Harofeh Med Ctr, Infect Dis Unit, Beer Yaagov, Israel

[8] Bnai Zion Med Ctr, Infect Dis & Control Unit, Haifa, Israel

[9] Beilinson Med Ctr, Rabin Med Ctr, Dept Emergency Med, Petah Tiqwa, Israel

[10] Tel Aviv Univ, Sackler Med SCh, Tel Aviv, Israel

[11] Technion Israel Inst Technol, Rappaport Fac Med, Haifa, Israel

来源：

EUROSURVEILLANCE | 2022年 / 27卷 / 35期

关键词：

D O I：

10.2807/1560-7917.ES.2022.27.35.2200636

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The current monkeypox virus global spread and tack of data regarding clinical specimens' infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq >= 35, <= 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox.

引用

页数：5

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