γ-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells

被引：49
作者：
Gallily, R
Even-Chen, T
Katzavian, G
Lehmann, D
Dagan, A
Mechoulam, R
机构：
[1] Hebrew Univ Jerusalem, Fac Med, Lautenberg Ctr Gen & Tumor Immunol, IL-1120 Jerusalem, Israel
[2] Hebrew Univ Jerusalem, Fac Med, Interdepartmental Equipment Unit, IL-1120 Jerusalem, Israel
[3] Hebrew Univ Jerusalem, Fac Med, Dept Biochem, IL-1120 Jerusalem, Israel
[4] Hebrew Univ Jerusalem, Fac Med, Dept Med Chem & Nat Prod, IL-1120 Jerusalem, Israel
来源：
LEUKEMIA & LYMPHOMA | 2003年 / 44卷 / 10期
基金：
以色列科学基金会;
关键词：
apoptosis; acute myeloid leukemia; HL-60; anandamide; cannabidiol; caspase;
D O I：
10.1080/1042819031000103917
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 mug/ml CBD and 15 mug/ml CBD-DMH, respectively, after a 24 h treatment. Prior exposure of the cells to gamma-irradiation (800 cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or gamma-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.
引用
收藏
页码：1767 / 1773
页数：7
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