Synthesis of 2,2-and 2,5-disubstituted 1-oxyl pyrrolidine radicals as new homobifunctional cross-linking spin labels

被引：10

作者：

Kálai, T

Jekö, J

Hubbell, WL

Hideg, K

机构：

[1] Univ Pecs, Inst Organ & Med Chem, H-7602 Pecs, Hungary

[2] ICN Hungary Ltd, H-4440 Tiszavasvari, Hungary

[3] Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

SYNTHESIS-STUTTGART | 2003年 / 13期

关键词：

cross linking reagents; proteins; free radicals; Grignard reactions; nitrones; lithium;

D O I：

10.1055/s-2003-41053

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The synthesis of 2,2- and 2,5-disubstituted pyrrolidine nitroxide radicals starting from readily available nitrones 1, 11 is described. The stable radicals are acylating (10, 20), alkylating (7, 17) and thiol-specific (8, 18) reagents capable of producing crosslinks in proteins over distances in the range of 10-15 Angstrom.

引用

页码：2084 / 2088

页数：5

共 27 条

[1] A NOVEL REVERSIBLE THIOL-SPECIFIC SPIN LABEL - PAPAIN ACTIVE-SITE LABELING AND INHIBITION [J].