Gene expression profiling identifies Hes6 as a transcriptional target of ATOH1 in cochlear hair cells

被引：15

作者：

Scheffer, Deborah

Sage, Cyrille

Corey, David P.

Pingault, Veronique ^{[1
]}

机构：

[1] INSERM, Equipe Avenir, IMRB, U841, F-94010 Creteil, France

[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Neurobiol, Boston, MA 02115 USA

来源：

FEBS LETTERS | 2007年 / 581卷 / 24期

关键词：

ear; inner; hair cells; ATOH1; protein; basic HelixLoop-Helix; transcription factors; hairy and enhancer of split 6 (Drosophila) protein; mouse;

D O I：

10.1016/j.febslet.2007.08.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATOH1 is a basic Helix-Loop-Helix transcription factor crucial for hair cell (HC) differentiation in the inner ear. In order to identify ATOH1 target genes, we performed a genome-wide expression profiling analysis in cells expressing ATOH1 under the control of a tetracycline-off system and found that HES6 expression is induced by ATOH1 We performed in situ hybridisation and showed that the rise and fall of Hes6 expression closely follow that of Atoh1 in cochlear HC. Moreover, electrophoretic mobility shift assays and luciferase assays show that ATOH1 activates HES6 transcription through binding to three clustered E boxes of its promoter. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4651 / 4656

页数：6

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