Circular RNA profile of breast cancer brain metastasis: identification of potential biomarkers and therapeutic targets

被引：54

作者：

Fu, Bo ^{[1
,2
]}

Zhang, Anqi ^{[1
]}

Li, Mingli ^{[3
]}

Pan, Li ^{[1
]}

Tang, Wenqiang ^{[1
]}

An, Meng ^{[4
]}

Liu, Wei ^{[1
]}

Zhang, Jianqiong ^{[5
]}

机构：

[1] Liaocheng Peoples Hosp, Dept Cent Lab, Liaocheng, Peoples R China

[2] Shandong Univ, Sch Basic Med Sci, Jinan, Shandong, Peoples R China

[3] Weifang Tradit Chinese Hosp, Dept Clin Lab, Weifang, Peoples R China

[4] Liaocheng Peoples Hosp, Dept Clin Lab, Liaocheng, Peoples R China

[5] Southeast Univ, Zhongda Hosp, Med Sch, Jiangsu Key Lab Mol Imaging & Funct Imaging, Nanjing, Jiangsu, Peoples R China

来源：

EPIGENOMICS | 2018年 / 10卷 / 12期

基金：

中国博士后科学基金;

关键词：

biomarker; breast cancer brain metastasis; circular RNA; expression profile; therapeutic target; CELLS; SUPPRESSES;

D O I：

10.2217/epi-2018-0090

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: To explore the circular RNA (circRNA) profile of breast cancer brain metastasis (BCBM). Materials & methods: RNA-seq was performed to identify the circRNA expression profile of brain metastatic breast cancer cell line 231-BR, in comparison with its parental nonspecific metastatic cell line MDA-MB-231. Results: A total of 215 upregulated and 191 downregulated circRNAs were identified. The expression levels of ten randomly selected majorly altered circRNAs were re-examined by real-time quantitative PCR and agarose gel electrophoresis, and the following alterations were verified: upregulation of hsa_circ_0001944, hsa_circ_0001481, hsa_circ_0000646, hsa_circ_0001006 and hsa_circ_0000732, and down-regulation of hsa_circ_0001910, hsa_circ_0008285 and hsa_circ_0000002. CircRNA/miRNA analysis revealed that hsa_circ_0001944 may be involved in BCBM through sponging up miR-509 and interfering with its binding to the downstream targets. Conclusion: This study provides a leading and fundamental circRNA profile of BCBM.

引用

页码：1619 / 1630

页数：12

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