Integration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization

被引：42

作者：

Xu, CH ^{[1
]}

Xia, GM ^{[1
]}

Zhi, DY ^{[1
]}

Xiang, FN ^{[1
]}

Chen, HM ^{[1
]}

机构：

[1] Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China

来源：

PLANT SCIENCE | 2003年 / 165卷 / 05期

基金：

中国国家自然科学基金;

关键词：

wheat; maize; asymmetric somatic hybridization; nuclear genome; cytogenome; integration;

D O I：

10.1016/S0168-9452(03)00287-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protoplasts were isolated from cultured cells of wheat and maize and fused using PEG. Calli and green plants were regenerated following irradiation of the maize, and some tested positive for hybridity using morphological, isozymic and various DNA-based marker systems. Genomic in situ hybridization (GISH) of selected maize-carrying regenerants showed that some maize chromatin was dispersed throughout the wheat nuclear genome. Cytogenomic analysis, using RFLP and SSR, demonstrated the presence of both wheat and maize loci in the mitochondrial genome, so that, in the hybrid individuals, either distinct mitochondria from each parent coexist and/or recombinant mitochondria have been generated. With respect to the chloroplast genome, there was no evidence of the presence of any introgression from maize. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1001 / 1008

页数：8

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