Molecular and biomechanical characterization of mineralized tissue by dental pulp cells on titanium

被引：52
作者：
Nakamura, H [1 ]
Saruwatari, L [1 ]
Aita, H [1 ]
Takeuchi, K [1 ]
Ogawa, T [1 ]
机构：
[1] Univ Calif Los Angeles, Sch Dent, Jane & Jerry Weintraub Ctr Reconstruct Biotechnol, Div Adv Prosthodont Biomat & Hosp Dent, Los Angeles, CA 90095 USA
来源：
JOURNAL OF DENTAL RESEARCH | 2005年 / 84卷 / 06期
关键词：
osseointegration; bone-titanium integration; nano-indentation; dental pulp stem cell; dual-acid-etched surface;
D O I：
10.1177/154405910508400606
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The application of implant therapy is still limited, because of various risk factors and the long healing time required for bone-titanium integration. This study explores the potential for osseointegration engineering with dental pulp cells (DPCs) by testing a hypothesis that DPCs generate mineralized tissue on titanium. DPCs extracted from rat incisors positive for CD44, alkaline phosphatase activity, and mineralizing capability were cultured on polystyrene and on machined and dual-acid-etched (DAE) titanium. Tissue cultured on titanium with a Ca/P ratio of 1.4 exhibited plate-like morphology, while that on the polystyrene exhibited fibrous and punctate structures. Tissues cultured on titanium were harder than those on polystyrene, 1.5 times on the machined and 3 times on the DAE. Collagen I, osteopontin, and osteocalcin genes were upregulated on titanium, especially the DAE surface. In conclusion, DPCs showing some characteristics of the previously identified dental pulp stem cells can generate mineralized tissue on titanium via the osteoblastic phenotype, which can be enhanced by titanium surface roughness.
引用
收藏
页码：515 / 520
页数：6
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