IκBζ is a key player in the antipsoriatic effects of secukinumab

Background: I kappa B zeta plays a key role in psoriasis by mediating IL-17A-driven effects, but the molecular mechanism by which IL-17A regulates I kappa B zeta expression is not clarified. Objective: We sought to explore the molecular transformation in patients with psoriasis during anti-IL-17A (secukinumab) treatment with a focus on I kappa B zeta. Methods: The study was an open-label, single-arm, single-center secukinumab treatment study that included 14 patients with plaque psoriasis. Skin biopsy specimens and blood samples were collected on days 0, 4,14, 42, and 84 and processed for microarray gene expression analysis. Furthermore, in vitro experiments with human keratinocytes and synovial fibroblasts were conducted. Results: Secukinumab improved clinical scores and histologic psoriasis features. Moreover, secukinumab altered the skin transcriptome. The major transcriptional shift appeared between day 14 and day 42 after treatment initiation, although 80 genes were differentially expressed already at day 4. Expression of nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor (I kappa B) zeta (NFKBIZ, the gene encoding I kappa B zeta) was reduced already after 4 days of treatment in the skin. NFKBIZ expression correlated to Psoriasis Area and Severity Index score, and NFKBIZ mRNA levels in the skin decreased during anti-IL-17A treatment. Moreover, specific NFKBIZ signature genes were significantly altered during antiIL-17A treatment. Finally, we identified NF-kappa B activator 1 (Act1), p38 mitogen-activated protein kinase (MAPK), Jun NH2-terminal kinase (JNK), and nuclear factor kappa-light- chain-enhancer of activated B cells (NF-kappa B) as key signaling pathways in NFKBIZ/I kappa B zeta regulation. Conclusion: Our results define a crucial role for I kappa B zeta in the antipsoriatic effect of secukinumab. Because I kappa B zeta signature genes were regulated already after 4 days of treatment, this strongly indicates that I kappa B zeta plays a crucial role in the antipsoriatic effects mediated by anti-IL-17A treatment.