Protein mechanical unfolding: A model with binary variables

被引:21
|
作者
Imparato, A.
Pelizzola, A.
Zamparo, M.
机构
[1] Politecn Torino, Dipartimento Fis, I-10129 Turin, Italy
[2] Politecn Torino, CNISM, I-10129 Turin, Italy
[3] Ist Nazl Fis Nucl, Sez Torino, I-10125 Turin, Italy
来源
JOURNAL OF CHEMICAL PHYSICS | 2007年 / 127卷 / 14期
关键词
D O I
10.1063/1.2776271
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A simple model, recently introduced as a generalization of the Wako-Saito model of protein folding, is used to investigate the properties of widely studied molecules under external forces. The equilibrium properties of the model proteins, together with their energy landscape, are studied on the basis of the exact solution of the model. Afterwards, the kinetic response of the molecules to a force is considered, discussing both force clamp and dynamic loading protocols and showing that theoretical expectations are verified. The kinetic parameters characterizing the protein unfolding are evaluated by using computer simulations and agree nicely with experimental results, when these are available. Finally, the extended Jarzynski equality is exploited to investigate the possibility of reconstructing the free energy landscape of proteins with pulling experiments. (C) 2007 American Institute of Physics.
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页数:11
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