A putative HCO-3 transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

被引：54
作者：
Bonfil, DJ
Ronen-Tarazi, M
Sültemeyer, D
Lieman-Hurwitz, J
Schatz, D
Kaplan, A [1 ]
机构：
[1] Hebrew Univ Jerusalem, Moshe Shilo Ctr, Dept Plant Sci, IL-91904 Jerusalem, Israel
[2] Hebrew Univ Jerusalem, Avron Evenari MInerva Ctr, IL-91904 Jerusalem, Israel
[3] Universitat Kaiserslautern, Fachbereich Biol, D-67653 Kaiserslautern, Germany
来源：
FEBS LETTERS | 1998年 / 430卷 / 03期
基金：
以色列科学基金会;
关键词：
CO2-concentrating mechanism; crossover; cyanobacteria; HCO3-; uptake; ictB;
D O I：
10.1016/S0014-5793(98)00662-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells, An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2, IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3- uptake in the wild type and IL-2 suggested the presence of two or three HCO3- carriers exhibiting different affinities to HCO3-. (C) 1998 Federation of European Biochemical Societies.
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页码：236 / 240
页数：5
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