A putative HCO-3 transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

被引：54

作者：

Bonfil, DJ

Ronen-Tarazi, M

Sültemeyer, D

Lieman-Hurwitz, J

Schatz, D

Kaplan, A ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Moshe Shilo Ctr, Dept Plant Sci, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Avron Evenari MInerva Ctr, IL-91904 Jerusalem, Israel

[3] Universitat Kaiserslautern, Fachbereich Biol, D-67653 Kaiserslautern, Germany

来源：

FEBS LETTERS | 1998年 / 430卷 / 03期

基金：

以色列科学基金会;

关键词：

CO2-concentrating mechanism; crossover; cyanobacteria; HCO3-; uptake; ictB;

D O I：

10.1016/S0014-5793(98)00662-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells, An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2, IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3- uptake in the wild type and IL-2 suggested the presence of two or three HCO3- carriers exhibiting different affinities to HCO3-. (C) 1998 Federation of European Biochemical Societies.

引用

页码：236 / 240

页数：5

共 29 条

[1] THE CO2 CONCENTRATING MECHANISM IN CYANOBACTERIA AND MICROALGAE [J].