High spatiotemporal resolution and low photo-toxicity fluorescence imaging in live cells and in vivo

被引:17
作者
Peng, Xiaohong [1 ,2 ]
Huang, Xiaoshuai [2 ]
Du, Ke [2 ]
Liu, Huisheng [1 ]
Chen, Liangyi [2 ,3 ]
机构
[1] Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Biol Sci & Med Engn, Interdisciplinary Innovat Inst Med & Engn, Beijing 100191, Peoples R China
[2] Peking Univ, Inst Mol Med, State Key Lab Membrane Biol, Beijing Key Lab Cardiometab Mol Med, Beijing 100871, Peoples R China
[3] PKU IDG, McGovern Inst Brain Res, Beijing 100871, Peoples R China
基金
北京市自然科学基金; 中国国家自然科学基金; 中国博士后科学基金;
关键词
STRUCTURED ILLUMINATION MICROSCOPY; MULTIPHOTON MICROSCOPY; ADAPTIVE OPTICS; 2-PHOTON MICROSCOPY; SUPER-RESOLUTION; STED MICROSCOPY; GENE-EXPRESSION; REAL-TIME; LONG-TERM; NANOSCOPY;
D O I
10.1042/BST20190020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Taking advantage of high contrast and molecular specificity, fluorescence microscopy has played a critical role in the visualization of subcellular structures and function, enabling unprecedented exploration from cell biology to neuroscience in living animals. To record and quantitatively analyse complex and dynamic biological processes in real time, fluorescence microscopes must be capable of rapid, targeted access deep within samples at high spatial resolutions, using techniques including super-resolution fluorescence microscopy, light sheet fluorescence microscopy, and multiple photon microscopy. In recent years, tremendous breakthroughs have improved the performance of these fluorescence microscopies in spatial resolution, imaging speed, and penetration. Here, we will review recent advancements of these microscopies in terms of the trade-off among spatial resolution, sampling speed and penetration depth and provide a view of their possible applications.
引用
收藏
页码:1635 / 1650
页数:16
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