Lambda-carrageenan treatment exacerbates the severity of cerebral malaria caused by Plasmodium berghei ANKA in BALB/c mice

被引:6
作者
Recuenco, Frances C. [1 ,2 ,4 ]
Takano, Ryo [2 ]
Chiba, Shiori [3 ]
Sugi, Tatsuki [1 ,2 ]
Takemae, Hitoshi [2 ]
Murakoshi, Fumi [1 ,2 ]
Ishiwa, Akiko [1 ,2 ]
Inomata, Atsuko [1 ]
Horimoto, Taisuke [1 ]
Kobayashi, Yoshiyasu [3 ]
Horiuchi, Noriyuki [3 ]
Kato, Kentaro [1 ,2 ]
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Microbiol, Bunkyo Ku, Tokyo 1138657, Japan
[2] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[3] Obihiro Univ Agr & Vet Med, Dept Basic Vet Med, Obihiro, Hokkaido 0808555, Japan
[4] Univ Philippines Los Banos, Coll Vet Med, Dept Vet Paraclin Sci, Laguna 4031, Philippines
来源
MALARIA JOURNAL | 2014年 / 13卷
基金
日本科学技术振兴机构;
关键词
Cerebral malaria; lambda-carrageenan; BALB/c mouse; Intracerebral haemorrhage; Plasmodium berghei ANKA; BLOOD-BRAIN-BARRIER; INDUCED INFLAMMATORY PAIN; SEQUESTRATION; EXPRESSION; FALCIPARUM; PATHOLOGY; INHIBIT; MODEL;
D O I
10.1186/1475-2875-13-487
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: There is an urgent need to develop and test novel compounds against malaria infection. Carrageenans, sulphated polysaccharides derived from seaweeds, have been previously shown to inhibit Plasmodium falciparum in vitro. However, they are inflammatory and alter the permeability of the blood-brain barrier, raising concerns that their use as a treatment for malaria could lead to cerebral malaria (CM), a severe complication of the disease. In this work, the authors look into the effects of the administration of.-carrageenan to the development and severity of CM in BALB/c mice, a relatively non-susceptible model, during infection with the ANKA strain of Plasmodium berghei. Methods: Five-week-old female BALB/c mice were infected with P. berghei intraperitoneally. One group was treated with.-carrageenan (PbCGN) following the 4-day suppressive test protocol, whereas the other group was not treated (PbN). Another group of healthy BALB/c mice was similarly given.-carrageenan (CGN) for comparison. The following parameters were assessed: parasitaemia, clinical signs of CM, and mortality. Brain and other vital organs were collected and examined for gross and histopathological lesions. Evans blue dye assays were employed to assess blood-brain barrier integrity. Results: Plasmodium berghei ANKA-infected BALB/c mice treated with.-carrageenan died earlier than those that received no treatment. Histopathological examination revealed that intracerebral haemorrhages related to CM were present in both groups of infected BALB/c mice, but were more numerous in those treated with.-carrageenan than in mock-treated animals. Inflammatory lesions were also observed only in the.-carrageenan-treated mice. These observations are consistent with the clinical signs associated with CM, such as head tilt, convulsions, and coma, which were observed only in this group, and may account for the earlier death of the mice. Conclusion: The results of this study indicate that the administration of.-carrageenan exacerbates the severe brain lesions and clinical signs associated with CM in BALB/c mice infected with P. berghei ANKA.
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页数:8
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