Engineering the Vasculature of Stem-Cell-Derived Liver Organoids

被引:8
|
作者
Zhang, Xv [1 ]
Tang, Liling [1 ]
Yi, Qian [2 ]
机构
[1] Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China
[2] Southwest Med Univ, Sch Basic Med Sci, Dept Physiol, Luzhou 646000, Peoples R China
关键词
liver organoids; stem cells; vascularization; maturation; endothelial cells; FUNCTIONAL HUMAN LIVER; ENDOTHELIAL-CELLS; HEPATOCYTES; EXPANSION; MOUSE; STAGE;
D O I
10.3390/biom11070966
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The vasculature of stem-cell-derived liver organoids can be engineered using methods that recapitulate embryonic liver development. Hepatic organoids with a vascular network offer great application prospects for drug screening, disease modeling, and therapeutics. However, the application of stem cell-derived organoids is hindered by insufficient vascularization and maturation. Here, we review different theories about the origin of hepatic cells and the morphogenesis of hepatic vessels to provide potential approaches for organoid generation. We also review the main protocols for generating vascularized liver organoids from stem cells and consider their potential and limitations in the generation of vascularized liver organoids.
引用
收藏
页数:10
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