Comparison of Conventional ELISA with Electrochemiluminescence Technology for Detection of Amyloid-β in Plasma

被引：39

作者：

Oh, Esther S. ^{[1
,2
,3
]}

Mielke, Michelle M. ^{[2
]}

Rosenberg, Paul B. ^{[2
]}

Jain, Alka

Fedarko, Neal S.

Lyketsos, Constantine G. ^{[2
]}

Mehta, Pankaj D. ^{[4
]}

机构：

[1] Johns Hopkins Univ, Dept Med, Div Geriatr Med & Gerontol, Sch Med, Baltimore, MD 21224 USA

[2] Johns Hopkins Univ, Sch Med, Dept Psychiat, Baltimore, MD 21224 USA

[3] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21224 USA

[4] New York State Inst Basic Res Dev Disabil, Dept Dev Neurobiol, Div Immunol, Staten Isl, NY 10314 USA

来源：

JOURNAL OF ALZHEIMERS DISEASE | 2010年 / 21卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Amyloid-beta protein; biological markers; enzyme-linked immunosorbent assay; plasma; MILD COGNITIVE IMPAIRMENT; ALZHEIMERS-DISEASE; ASSOCIATION; DIAGNOSIS; PEPTIDES; PROTEIN; RISK;

D O I：

10.3233/JAD-2010-100456

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Plasma amyloid-beta (A beta) level could be useful as a non-invasive biomarker in Alzheimer's disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma A beta quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for this may include the differences in the affinities of antibodies, and purity and source of A beta peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.

引用

页码：769 / 773

页数：5

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