Binding of an aptamer to the N-terminal fragment of VCAM-1

被引：14
作者：
Chauveau, Fabien
Aissouni, Youssef
Hamm, Jorg
Boutin, Herve
Libri, Domenico
Duconge, Frederic
Tavitian, Bertrand
机构：
[1] CEA, DSV, IBM, SHFJ,Lab Imag Mol Expt, F-91400 Orsay, France
[2] CNRS, Ctr Genet Mol, Gif Sur Yvette, France
[3] Univ Turin, Mol Biotechnol Ctr, I-10124 Turin, Italy
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2007年 / 17卷 / 22期
关键词：
SELEX; VCAM-1; aptamer;
D O I：
10.1016/j.bmcl.2007.09.046
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
In vitro selection of 2 '-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10 nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay. (C) 2007 Elsevier Ltd. All rights reserved.
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收藏
页码：6119 / 6122
页数：4
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