Raf-1 is a binding partner of DSCR

被引：27
作者：
Cho, YJ
Abe, M
Kim, SY
Sato, Y [1 ]
机构：
[1] Tohoku Univ, Inst Dev Aging & Canc, Dept Vasc Biol, Sendai, Miyagi 9808575, Japan
[2] Catholic Univ Korea, Coll Med, Dept Pharmacol, Seoul 137701, South Korea
[3] Catholic Univ Korea, Coll Med, Cell Death Dis Ctr MRC, Seoul 137701, South Korea
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2005年 / 439卷 / 01期
关键词：
DSCRI; Raf-1; endothelial cell; angiogenesis;
D O I：
10.1016/j.abb.2005.05.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Down syndrome critical region 1 (DSCR1) is recognized as an endogenous calcineurin inhibitor. DSCR1 is induced in endothelial cells and may play an important role in inflammation and angiogenesis. To address a novel function of DSCR1, we searched interacting partners of DSCR1. We performed pull-down analysis using DSCR1 as a bait and identified Raf-1 as a binding partner. The association of Raf-1 was confirmed by co-immunoprecipitation in GM7373 cells expressing green fluorescence protein tagged DSCR1. We determined two Raf-1 binding regions in DSCR1; one in the N-terminus and the other in the C-terminus regions. We further demonstrated that calpain cleaved DSCR1 and generated fragments with different binding affinity to Raf-1 or calcineurin. These results constitute the first demonstration of Raf-1 as a binding partner of DSCR1, and suggest a novel role of DSCR1. (C) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：121 / 128
页数：8
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