Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors

被引：155
作者：
Wan, Yi [1 ,2 ]
Wang, Yi [1 ]
Wu, Jiajia [1 ,2 ]
Zhag, Dun [1 ]
机构：
[1] Chinese Acad Sci, Shandong Prov Key Lab Corros Sci, Inst Oceanol, Qingdao 266071, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 03期
基金：
中国国家自然科学基金;
关键词：
BIOFUNCTIONAL MAGNETIC NANOPARTICLES; RESONANCE ENERGY-TRANSFER; BACTERIA; IDENTIFICATION; DNA;
D O I：
10.1021/ac103047c
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 x 10(2) to 1.8 x 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfite-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated,silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
引用
收藏
页码：648 / 653
页数：6
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