Mitochondrial DNA enrichment for species identification and evolutionary analysis

被引:42
作者
Burgener, M [1 ]
Hubner, P [1 ]
机构
[1] Univ Bern, Dept Chem & Biochem, Food Chem Lab, CH-3012 Bern, Switzerland
来源
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY | 1998年 / 207卷 / 04期
关键词
mitochondrial DNA; polymerase chain reaction; species identification; evolutionary analysis; food authenticity;
D O I
10.1007/s002170050329
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Nucleic acid-based species identification often targets the mitochondrial encoded cytb gene. However, polymerase chain reaction (PCR)- restriction fragment length polymorphism (RFLP) analysis using universal primers sometimes leads to ambiguous results, which are due to the presence of nuclear encoded pseudo-cytb genes. Such ambiguities were succesfully avoided using a newly developed method for the enrichment of mitochondrial DNA. In addition, a mitochondrial cyrb-specific PCR system was designed allowing the unambiguous identification of game meat.
引用
收藏
页码:261 / 263
页数:3
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