Biocompatible antimicrobial electrospun nanofibers functionalized with ε-poly-L-lysine

被引:39
作者
Amariei, Georgiana [1 ]
Kokol, Vanja [2 ]
Vivod, Vera [2 ]
Boltes, Karina [1 ]
Leton, Pedro [1 ]
Rosal, Roberto [1 ]
机构
[1] Univ Alcala, Dept Chem Engn, E-28871 Madrid, Spain
[2] Univ Maribor, Inst Engn Mat & Design, SI-2000 Maribor, Slovenia
关键词
Electrospun fibers; epsilon-Poly(L-lysine); Antibacterial materials; Biocompatible dressings; DRUG-DELIVERY SYSTEMS; WOUND DRESSINGS; ANTIBACTERIAL ACTIVITY; ALPHA-HELIX; POLYLYSINE; MECHANISM; SURFACE; POLY(L-LYSINE); TRANSITION; CHEMISTRY;
D O I
10.1016/j.ijpharm.2018.10.037
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The antimicrobial polypeptide epsilon-poly(L-lysine) (epsilon-PL) was electrostatically incorporated to poly(acrylic acid) (PAA)/poly(vinyl alcohol) (PVA) electrospun nanofibers. epsilon-PL loading and distribution was assessed by infrared spectra, zeta-potential measurements and the primary amino reactive dye fluorescamine. Functionalized fibers with 485 +/- 140 nm diameter, could be loaded with 0.57-0.74 g epsilon-PL (g dressing)(-1) that released at a constant rate of 5.4 +/- 2.8 mg epsilon-PL (g dressing day)(-1). Such a dressings resulted in two orders of magnitude lower bacterial colonization than non-functionalized PAA-PVA after 14 days of incubation. Bacterial impairment was attributed to the damage of cell membranes and the formation of intracellular reactive oxygen species. epsilon-PL functionalized nanofibers did not display cytotoxicity to human corneal epithelial cells, HCEpC, in 24 h MTT assays. However, the viability of rapidly growing tumoral HeLa cells decreased > 50% under the same conditions. The prepared biocompatible nanofibrous dressings with durable antibacterial activity show potential application as wound dressings and other biomedical uses.
引用
收藏
页码:141 / 148
页数:8
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