Point Mutations in FgSdhC2 or in the 5′ Untranslated Region of FgSdhC1 Confer Resistance to a Novel Succinate Dehydrogenase Inhibitor Flubeneteram in Fusarium graminearum

被引：30

作者：

Chen, Wenchan ^{[1
,2
]}

Wei, Lingling ^{[1
]}

Li, Xiujuan ^{[1
]}

Ma, Hongyu ^{[1
]}

Lou, Tiancheng ^{[1
]}

Zhang, Pengcheng ^{[1
]}

Zheng, Huanhuan ^{[1
]}

Zhu, Xiaolei ^{[3
]}

Zhang, Yu ^{[4
]}

Liu, Fengquan ^{[2
]}

Chen, Changjun ^{[1
]}

Yang, Guangfu ^{[3
]}

机构：

[1] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

[3] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China

[4] Zhejiang A&F Univ, Coll Adv Agr Sci, Hangzhou 311300, Zhejiang, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2021年 / 69卷 / 44期

基金：

中国国家自然科学基金;

关键词：

Fusarium graminearum; flubeneteram; 5 ' UTR; mutations; resistance mechanism; IRON-SULFUR SUBUNIT; BOTRYTIS-CINEREA; MOLECULAR CHARACTERIZATION; BOSCALID RESISTANCE; SDHI FUNGICIDES; 14-ALPHA-DEMETHYLASE GENE; WHEAT; SENSITIVITY; MECHANISMS; ASIATICUM;

D O I：

10.1021/acs.jafc.1c04363

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Fusarium graminearum is one of the phytopathogenic fungi causing cereal fusarium head blight worldwide. Flubeneteram (Flu) is a novel succinate dehydrogenase inhibitor (SDHI) which exhibits strong fungicidal activity against F. graminearum. In this study, four Flu-resistant (FluR) mutants were generated by fungicide domestication from the wildtype strain PH-1. Sequencing alignment results of FgSdh from PH-1 and FluR mutants showed that all the mutations could be categorized into three resistant genotypes. Genotype I had an A-to-T mutation at the -57 bp of the 5' untranslated region (5' UTR) of FgSdhC(1), while genotypes II and III carried nonsynonymous mutations conferring T77I or R86C in FgSdhC(2), respectively. All the mutations conferring the Flu resistance and causing fitness penalty were validated. The genotype I mutant showed high Flu-resistance, while genotype II and III mutants exhibited low Flu resistance. Additionally, all the FluR genotypes showed distinct cross-resistance patterns among the five SDHIs.

引用

页码：13006 / 13019

页数：14

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