Novel strategy in Trypanosoma cruzi cell invasion:: Implication of cholesterol and host cell microdomains

被引:56
作者
Fernandes, Maria Cecilia
Cortez, Mauro
Geraldo Yoneyama, Kelly Aparecida
Straus, Anita Hilda
Yoshida, Nobuko
Mortara, Renato Arruda
机构
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, BR-04023062 Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Trypanosoma cruzi; trypomastigote; amastigote; membrane rafts; cholesterol; cell invasion;
D O I
10.1016/j.ijpara.2007.04.025
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Trypanosoma cruzi, the etiological agent of Chagas' disease, is an obligatory intracellular parasite in the mammalian host. In order to invade a wide variety of mammalian cells, T cruzi engages parasite components that are differentially expressed among strains and infective forms. Because the identification of putative protein receptors has been particularly challenging, we investigated whether cholesterol and membrane rafts, sterol- and sphingolipid-enriched membrane domains, could be general host surface components involved in invasion of metacyclic trypomastigotes and extracellular amastigotes of two parasite strains with distinct infectivities. HeLa or Vero cells treated with methyl-beta-cyclodextrin (M beta CD) are less susceptible to invasion by both infective forms, and the effect was dose-dependent for trypomastigote but not amastigote invasion. Moreover, treatment of parasites with MPCD only inhibited trypomastigote invasion. Filipin labeling confirmed that host cell cholesterol concentrated at the invasion sites. Binding of a cholera toxin B subunit (CTX-B) to ganglioside GM1, a marker of membrane rafts, inhibited parasite infection. Cell labeling with CTX-B conjugated to fluorescein isothiocyanate revealed that not only cholesterol but also GM1 is implicated in parasite entry. These findings thus indicate that microdomains present in mammalian cell membranes, that are enriched in cholesterol and GM1, are involved in invasion by T cruzi infective forms. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1431 / 1441
页数:11
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