Inteins as Traceless Purification Tags for Unnatural Amino Acid Proteins

被引：28

作者：

Batjargal, Solongo ^{[1
]}

Walters, Christopher R. ^{[1
]}

Petersson, E. James ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 05期

基金：

美国国家卫生研究院;

关键词：

GENETIC-CODE; ESCHERICHIA-COLI; ENERGY-TRANSFER;

D O I：

10.1021/ja5103019

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The expression of proteins containing unnatural amino acids through suppression of a stop codon can be limited by truncation due to competition with release factors. When the site of incorporation is near the C-terminus, it may not be feasible to separate the full-length unnatural amino acid protein from the truncated form. We report a simple, traceless procedure that allows one to isolate the desired protein using a C-terminal intein fusion.

引用

页码：1734 / 1737

页数：4

共 16 条

[11] Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Forster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies [J].