Purification and Characterization of an α-Galactosidase from Aspergillus fumigatus

被引：21

作者：

de Rezende, ST ^{[1
]}

Guimaraes, VM

Rodrigues, MD

Felix, CR

机构：

[1] Univ Fed Vicosa, Dept Bioquim & Biol Mol, BR-36571000 Vicosa, MG, Brazil

[2] Univ Brasilia, Dept Biol Celular, BR-70910900 Brasilia, DF, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2005年 / 48卷 / 02期

关键词：

Aspergillus fumigatus; alpha-galactosidase; raffinose oligosaccharides;

D O I：

10.1590/S1516-89132005000200005

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by; gel filtration and two ion exchange chromatographies and showed Mw of 54. 7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 degrees C, and retained about 80% of the original activity after incubation for 90 minutes at 50 degrees C The K-M for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate.

引用

页码：195 / 202

页数：8

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