Mapping Quantitative Trait Loci for Tolerance to Pythium irregulare in Soybean (Glycine max L.)

被引:15
作者
Lin, Feng [1 ]
Wani, Shabir H. [1 ,2 ]
Collins, Paul J. [1 ]
Wen, Zixiang [1 ]
Gu, Cuihua [1 ]
Chilvers, Martin I. [1 ]
Wang, Dechun [1 ]
机构
[1] Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St,Rm A384-E, E Lansing, MI 48824 USA
[2] Sher E Kashmir Univ Agr Sci & Technol Kashmir, Mt Res Ctr Field Crops, Anantnag 192101, J&K, India
来源
G3-GENES GENOMES GENETICS | 2018年 / 8卷 / 10期
基金
美国食品与农业研究所;
关键词
Pythium irregulare; Quantitative Trait Loci Mapping; Single Nucleotide Polymorphism; DAMPING-OFF; PHYTOPHTHORA-SOJAE; RESISTANCE QTL; LINKAGE MAPS; SEED ROT; AGGRESSIVENESS; APHANIDERMATUM; SENSITIVITY; GENES; CORN;
D O I
10.1534/g3.118.200368
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Pythium root rot is one of the significant diseases of soybean (Glycine max (L.) Merr.) in the United States. The causal agent of the disease is a soil-borne oomycete pathogen Pythium irregulare, the most prevalent and aggressive species of Pythium in North Central United States. However, few studies have been conducted in soybean for the identification of quantitative trait loci (QTL) for tolerance to P. irregulare. In this study, two recombinant inbred line (RIL) populations (designated as POP1 and POP2) were challenged with P. irregulare (isolate CMISO2-5-14) in a greenhouse assay. POP1 and POP2 were derived from 'E09014' x 'E05226-T' and 'E05226-T' x 'E09088', and contained 113 and 79 lines, respectively. Parental tests indicated that 'E05226-T' and 'E09014' were more tolerant than 'E09088', while 'E09088' was highly susceptible to the pathogen. The disease indices, root weight of inoculation (RWI) and ratio of root weight (RRW) of both populations showed near normal distributions, with transgressive segregation, suggesting the involvement of multiple QTL from both parents contributed to the tolerance. All the lines were genotyped using Illumina Infinium BARCSoySNP6K iSelect BeadChip and yielded 1373 and 1384 polymorphic markers for POP1 and POP2, respectively. Notably, despite high density, polymorphic markers coverage was incomplete in some genomic regions. As such, 28 and 37 linkage groups were obtained in POP1 and POP2, respectively corresponding to the 20 soybean chromosomes. Using RRW, one QTL was identified in POP1 on Chromosome 20 that explained 12.7-13.3% of phenotypic variation. The desirable allele of this QTL was from 'E05226-T'. Another QTL was found in POP2 on Chromosome 11. It explained 15.4% of the phenotypic variation and the desirable allele was from 'E09088'. However, no QTL were identified using RWI in either population. These results supported that RRW was more suitable to be used to evaluate P. irregulare tolerance in soybean.
引用
收藏
页码:3155 / 3161
页数:7
相关论文
共 31 条
  • [21] Construction of high resolution genetic linkage maps to improve the soybean genome sequence assembly Glyma1.01
    Song, Qijian
    Jenkins, Jerry
    Jia, Gaofeng
    Hyten, David L.
    Pantalone, Vince
    Jackson, Scott A.
    Schmutz, Jeremy
    Cregan, Perry B.
    [J]. BMC GENOMICS, 2016, 17
  • [22] Development and Evaluation of SoySNP50K, a High-Density Genotyping Array for Soybean
    Song, Qijian
    Hyten, David L.
    Jia, Gaofeng
    Quigley, Charles V.
    Fickus, Edward W.
    Nelson, Randall L.
    Cregan, Perry B.
    [J]. PLOS ONE, 2013, 8 (01):
  • [23] High-Density Mapping of Resistance QTL Toward Phytophthora sojae, Pythium irregulare, and Fusarium graminearum in the Same Soybean Population
    Stasko, Anna K.
    Wickramasinghe, Damitha
    Nauth, Brittany J.
    Acharya, Bhupendra
    Ellis, Margaret L.
    Taylor, Christopher G.
    McHale, Leah K.
    Dorrance, Anne E.
    [J]. CROP SCIENCE, 2016, 56 (05) : 2476 - 2492
  • [24] Characterization of Seed Rot Resistance to Pythium aphanidermatum in Soybean
    Urrea, Keiddy
    Rupe, John
    Chen, Pengyin
    Rothrock, Craig S.
    [J]. CROP SCIENCE, 2017, 57 (03) : 1394 - 1403
  • [25] MapChart: Software for the graphical presentation of linkage maps and QTLs
    Voorrips, RE
    [J]. JOURNAL OF HEREDITY, 2002, 93 (01) : 77 - 78
  • [26] Wang S. C. J. B., 2012, Windows QTL Cartographer 2.5
  • [27] Pathogenicity of Pythium species causing seed rot and damping-off in soybean under controlled conditions
    Wei, Lai
    Xue, Allen G.
    Cober, Elroy R.
    Babcock, Carolyn
    Zhang, Jinxiu
    Zhang, Shuzhen
    Li, Wenbin
    Wu, Junjiang
    Liu, Lijun
    [J]. PHYTOPROTECTION, 2010, 91 : 3 - 10
  • [28] Genome-wide association mapping of quantitative resistance to sudden death syndrome in soybean
    Wen, Zixiang
    Tan, Ruijuan
    Yuan, Jiazheng
    Bales, Carmille
    Du, Wenyan
    Zhang, Shichen
    Chilvers, Martin I.
    Schmidt, Cathy
    Song, Qijian
    Cregan, Perry B.
    Wang, Dechun
    [J]. BMC GENOMICS, 2014, 15
  • [29] Wrather J., 2009, PLANT HLTH PROG, DOI DOI 10.1094/PHP-2009-0401-01-RS
  • [30] Mapping novel aphid resistance QTL from wild soybean, Glycine soja 85-32
    Zhang, Shichen
    Zhang, Zhongnan
    Bales, Carmille
    Gu, Cuihua
    DiFonzo, Chris
    Li, Ming
    Song, Qijian
    Cregan, Perry
    Yang, Zhenyu
    Wang, Dechun
    [J]. THEORETICAL AND APPLIED GENETICS, 2017, 130 (09) : 1941 - 1952