A moderately high level of dietary lipid inhibited the protein secretion function of liver in juvenile tiger puffer Takifugu rubripes

被引：26
作者：
Xu, Houguo [1 ,2 ]
Liao, Zhangbin [1 ]
Zhang, Qinggong [1 ]
Wei, Yuliang [1 ,2 ]
Liang, Mengqing [1 ,2 ]
机构：
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Peoples R China
来源：
AQUACULTURE | 2019年 / 498卷
基金：
中国国家自然科学基金;
关键词：
Takifugu rubripes; Diet; Lipid; Liver function; TILAPIA OREOCHROMIS-NILOTICUS; CARP CTENOPHARYNGODON-IDELLA; COD GADUS-MORHUA; NONSPECIFIC IMMUNE-RESPONSES; FATTY-ACID REQUIREMENTS; TURBOT PSETTA-MAXIMA; BLUNT SNOUT BREAM; FEED-UTILIZATION; BODY-COMPOSITION; GRASS CARP;
D O I：
10.1016/j.aquaculture.2018.08.033
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Tiger puffer have a unique lipid storage pattern. They store lipid predominantly in liver. To investigate the hepatic physiology of tiger puffer in response to a moderately high level of dietary lipid, a 74-day feeding trial was conducted, followed by a hepatic transcriptome assay. Two experimental diets which had an optimal (10.44%, Control) or moderately higher (14.64%, high-lipid diet, HL) dietary lipid level was formulated. Each diet was fed to triplicate tanks. No significant difference was observed in growth and feed efficiency between the two groups, but the survival was significantly lower in group HL. The HL diet also resulted in significantly higher lipid accumulation in liver, which was reflected in the hepatic histology. The biochemical parameters in serum confirmed the existence of stress in liver caused by high lipid intake. A total of 63 differentially expressed genes between groups were observed in the hepatic transcriptome assay, which were primarily enriched in Gene Ontology (GO) terms such as Endopeptidase activity, Secretion, and Organic acid transport, as well as in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways such as Protein processing in endoplasmic reticulum and DNA replication. The HL diet tended to inhibit the protein synthesis and secretion in liver, and this was evidenced by activities/concentrations of selected proteins secreted into intestine. Genes related to liver protection and repair were transcriptionally stimulated by diet HL. In conclusion, compared to the optimal lipid requirement, a moderately higher lipid level in the diet for tiger puffer did not compromise the growth performance, but tended to inhibit the protein secretion function of liver and stimulate the processes related to liver protection and repair. The present results will be beneficial to both feed management in tiger puffer farming and elucidation of lipid metabolism characteristics in fish which store lipid in liver.
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页码：17 / 27
页数：11
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