MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously

被引：72

作者：

Abrahamsson, Sara ^{[1
,2
,3
]}

McQuilken, Molly ^{[4
]}

Mehta, Shalin B. ^{[3
]}

Verma, Amitabh ^{[3
]}

Larsch, Johannes ^{[1
,2
]}

Ilic, Rob ^{[5
]}

Heintzmann, Rainer ^{[6
,7
,8
,9
]}

Bargmann, Cornelia I. ^{[1
,2
]}

Gladfelter, Amy S. ^{[4
]}

Oldenbourg, Rudolf ^{[3
]}

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10065 USA

[2] Rockefeller Univ, Lulu & Anthony Wang Lab Neural Circuits & Behav, New York, NY 10065 USA

[3] Marine Biol Lab, Woods Hole, MA 02543 USA

[4] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA

[5] Cornell Univ, Cornell NanoScale Sci & Technol Facil CNF, Ithaca, NY 14853 USA

[6] Leibniz Inst Photon Technol, Jena, Germany

[7] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[8] Univ Jena, Abbe Ctr Photon, Jena, Germany

[9] Kings Coll London, Randall Div Cell & Mol Biophys, London WC2R 2LS, England

来源：

OPTICS EXPRESS | 2015年 / 23卷 / 06期

基金：

美国国家科学基金会;

关键词：

FLUORESCENCE; BIREFRINGENCE; DYNAMICS;

D O I：

10.1364/OE.23.007734

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed an imaging system for 3D time-lapse polarization microscopy of living biological samples. Polarization imaging reveals the position, alignment and orientation of submicroscopic features in label-free as well as fluorescently labeled specimens. Optical anisotropies are calculated from a series of images where the sample is illuminated by light of different polarization states. Due to the number of images necessary to collect both multiple polarization states and multiple focal planes, 3D polarization imaging is most often prohibitively slow. Our MF-PolScope system employs multifocus optics to form an instantaneous 3D image of up to 25 simultaneous focal-planes. We describe this optical system and show examples of 3D multi-focus polarization imaging of biological samples, including a protein assembly study in budding yeast cells. (C) 2015 Optical Society of America

引用

页码：7734 / 7754

页数：21

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