A paradox: Insulin inhibits expression and secretion of resistin which induces insulin resistance

被引：15
作者：
Liu, Feng [1 ,2 ]
Fan, Hong-Qi [1 ]
Qiu, Jie [1 ]
Wang, Bin [1 ]
Zhang, Min [1 ]
Gu, Nan [1 ]
Zhang, Chun-Mei [1 ]
Fei, Li [1 ]
Pan, Xiao-Qing [1 ]
Guo, Mei [1 ]
Chen, Rong-Hua [1 ]
Guo, Xi-Rong [1 ,2 ]
机构：
[1] Nanjing Med Univ, Inst Pediat, Nanjing 210029, Jiangsu Prov, Peoples R China
[2] Nanjing Med Univ, Nanjing Matern & Child Hlth Hosp, Dept Pediat, Nanjing 210004, Jiangsu Prov, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2008年 / 14卷 / 01期
关键词：
resistin; insulin; insulin resistance;
D O I：
10.3748/wjg.14.95
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To confirm whether insulin regulates resistin expression and secretion during differentiation of 3T3-L1 preadipocytes and the relationship of resistin with insulin resistance both in vivo and in vitro. METHODS: Supernatant resistin was measured during differentiation of 3T3-L1 preadipocytes. L6 rat myoblasts and hepatoma cell line H4IIE were used to confirm the cellular function of resistin. Diet-induced obese rats were used as an insulin resistance model to study the relationship of resistin with insulin resistance. RESULTS: Resistin expression and secretion were enhanced during differentiation 3T3-L1 preadipocytes. This cellular differentiation stimulated resistin expression and secretion, but was suppressed by insulin. Resistin also induced insulin resistance in H4IIE hepatocytes and L6 myoblasts. In diet-induced obese rats, serum resistin levels were negatively correlated with insulin sensitivity, but not with serum insulin. CONCLUSION: Insulin can inhibit resistin expression and secretion in vitro, but insulin is not a major regulator of resistin in vivo. Fat tissue mass affects insulin sensitivity by altering the expression and secretion of resistin. 2008 WJG. All rights reserved.
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收藏
页码：95 / 100
页数：6
相关论文
共 30 条
[1]   Regulation of fasted blood glucose by resistin [J].
Banerjee, RR ;
Rangwala, SM ;
Shapiro, JS ;
Rich, AS ;
Rhoades, B ;
Qi, Y ;
Wang, J ;
Rajala, MW ;
Pocai, A ;
Scherer, PE ;
Steppan, CM ;
Ahima, RS ;
Obici, S ;
Rossetti, L ;
Lazar, MA .
SCIENCE, 2004, 303 (5661) :1195-1198
[2]   Regulation of leptin secretion from white adipocytes by insulin, glycolytic substrates, and amino acids [J].
Cammisotto, PG ;
Gélinas, Y ;
Deshaies, Y ;
Bukowiecki, LJ .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2005, 289 (01) :E166-E171
[3]   Globular adiponectin increases GLUT4 translocation and glucose uptake but reduces glycogen synthesis in rat skeletal muscle cells [J].
Ceddia, RB ;
Somwar, R ;
Maida, A ;
Fang, X ;
Bikopoulos, G ;
Sweeney, G .
DIABETOLOGIA, 2005, 48 (01) :132-139
[4]   Regulation of the mouse protein targeting to glycogen (PTG) promoter by the FoxA2 forkhead protein and by 3′,5′-cyclic adenosine 5′-monophosphate in H4IIE hepatoma cells [J].
Cheng, Alan ;
Zhang, Mei ;
Crosson, Sean M. ;
Bao, Zhao Q. ;
Saltiel, Alan R. .
ENDOCRINOLOGY, 2006, 147 (07) :3606-3612
[5]   LOSS OF HEPATIC AUTOREGULATION AFTER CARBOHYDRATE OVERFEEDING IN NORMAL MAN [J].
CLORE, JN ;
HELM, ST ;
BLACKARD, WG .
JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (04) :1967-1972
[6]   Suppression of preadipocyte differentiation and promotion of adipocyte death by HIV protease inhibitors [J].
Dowell, P ;
Flexner, C ;
Kwiterovich, PO ;
Lanes, MD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (52) :41325-41332
[7]  
El Naggar Enas Atef, 2004, Kobe Journal of Medical Sciences, V50, P39
[8]   Diabetes, lipids, and adipocyte secretagogues [J].
Faraj, M ;
Lu, HL ;
Cianflone, K .
BIOCHEMISTRY AND CELL BIOLOGY, 2004, 82 (01) :170-190
[9]   Elevation in tanis expression alters glucose metabolism and insulin sensitivity in H4IIE cells [J].
Gao, Y ;
Walder, K ;
Sunderland, T ;
Kantham, L ;
Feng, HC ;
Quick, M ;
Bishara, N ;
de Silva, A ;
Augert, G ;
Tenne-Brown, J ;
Collier, GR .
DIABETES, 2003, 52 (04) :929-934
[10]  
Gong HX, 2003, ACTA PHARMACOL SIN, V24, P569
123