Extraction, purification, characterization and hypoglycemic activity of a polysaccharide isolated from the root of Ophiopogon japonicus

被引：78

作者：

Chen, Xiaoming ^{[1
]}

Jin, Jing ^{[1
]}

Tang, Jia ^{[1
]}

Wang, Zhongfu ^{[2
]}

Wang, Junjun ^{[1
]}

Jin, Liqin ^{[1
]}

Lu, Jianxin ^{[1
]}

机构：

[1] Wenzhou Med Coll, Inst Glycobiol Engn, Zhejiang Prov Key Lab Med Genet, Wenzhou 325035, Peoples R China

[2] NW Univ Xian, Key Lab, Minist Educ Western China Resource Biol & Biotech, Coll Life Sci, Xian 710069, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 83卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Ophiopogon japonicus; Polysaccharide; Characterization; Hypoglycemic activity; STREPTOZOTOCIN; FR;

D O I：

10.1016/j.carbpol.2010.08.050

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this research a water-soluble polysaccharide (OJP1) extracted with hot water from the roots of Ophiopogon japonicus which is a traditional Chinese medicinal herb was precipitated with 95% ethanol and purified by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel filtration chromatography The high performance gel permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of OJP1 was 35 2 kDa Monosaccharides analysis revealed that the OJP1 is composed of Ara Glc Gal with a relative molar ratio of 1 16 8 Pharmaceutical experiments showed OJP1 can significantly reduce blood glucose level increase the insulin level and remediating destruction of pancreatic islets in STZ-induced diabetic rats compared with the diabetic control group The study shows that OJP1 has an anti-diabetic effect on STZ-induced diabetic rats and has potential use as an anti-diabetic agent Crown Copyright (C) 2010 Published by Elsevier Ltd All rights reserved

引用

页码：749 / 754

页数：6

共 25 条

[1] Molecular regulation of pancreatic β-cell mass development, maintenance, and expansion [J].