Two-Enzyme Hydrogen-Borrowing Amination of Alcohols Enabled by a Cofactor-Switched Alcohol Dehydrogenase

被引：66

作者：

Thompson, Matthew P. ^{[1
]}

Turner, Nicholas J. ^{[1
]}

机构：

[1] Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England

来源：

CHEMCATCHEM | 2017年 / 9卷 / 20期

关键词：

amination; biocatalysis; enzymes; hydrogen borrowing; protein engineering; SITE-DIRECTED MUTAGENESIS; COENZYME SPECIFICITY; PSEUDOMONAS-FLUORESCENS; ASYMMETRIC AMINATION; SECONDARY ALCOHOLS; SEC-ALCOHOLS; BINDING-SITE; TRANSAMINASES; RACEMIZATION; CONVERSION;

D O I：

10.1002/cctc.201701092

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The NADPH-dependent secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus (TeSADH), displaying broad substrate specificity and low enantioselectivity, was engineered to accept NADH as a cofactor. The engineered TeSADH showed a >10000-fold switch from NADPH towards NADH compared to the wildtype enzyme. This TeSADH variant was applied to a biocatalytic hydrogen-borrowing system that employed catalytic amounts of NAD(+), ammonia, and an amine dehydrogenase, which thereby enabled the conversion a range of alcohols into chiral amines.

引用

页码：3833 / 3836

页数：4

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