Characterization of herpes simplex virus type 1 thyraidine kinase mutants selected under a single round of high-dose brivudin

被引：37

作者：

Andrei, G ^{[1
]}

Balzarini, J ^{[1
]}

Fiten, P ^{[1
]}

De Clercq, E ^{[1
]}

Opdenakker, G ^{[1
]}

Snoeck, R ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Rega Inst Med Res, Virol Lab, B-3000 Louvain, Belgium

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 09期

关键词：

D O I：

10.1128/JVI.79.9.5863-5869.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A broad variety of herpes simplex virus type 1 clones was selected under a single round of high-dose selection with brivudin. Mutations in the thymidine kinase (TK) genes consisted of 42% frameshift mutations within homopolymer repeats of G's and C's and single nucleotide substitutions (58%) that produced stop codons (Q261 and R281) or a new codon at the site of the substitution (A168T, R51W, G59W, G206R, R220H, Y239S, and T287 M). The A168T change, associated with an altered TK phenotype, proved to be the most commonly selected substitution. For the different mutants, a correlation between phenotype, genotype, and in vivo neurovirulence was observed.

引用

页码：5863 / 5869

页数：7

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