Optimization of β-xylosidase recovery by reversed micelles using response surface methodology

beta-Xylosidase recovery by reversed micelles using CTAB cationic surfactant was performed under different experimental conditions. A 22-full orthogonal design with center points was employed to optimize the recovery of this enzyme and to evaluate the influence of the factors CTAB ( A) and butanol concentration ( B) on the enzyme extraction. A mathematical model to represent the enzyme recovery as a function of A and B was proposed. According to the model, the CTAB and butanol concentrations necessary to attain the maximum beta-xylosidase recovery (43%) were 0.26 M and 29.4%, respectively. The results showed that the recovery value predicted by the model was similar to that obtained experimentally ( 44.3%).