Optimization of β-xylosidase recovery by reversed micelles using response surface methodology

被引：0
作者：
Hasmann, FA
Cortez, DV
Pessoa, A
Roberto, IC
机构：
[1] Univ Sao Paulo, Inst Pesquisas Tecnol Butantan, Sao Paulo, Brazil
[2] Fac Engn Quim Lorena, Dept Biotecnol, Sao Paulo, Brazil
[3] Univ Sao Paulo, Dept Tecnol Bioquim Farmaceut, Sao Paulo, Brazil
来源：
ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2003年 / 6卷 / 02期
关键词：
beta-xylosidase; experimental design; liquid-liquid extraction; optimization; reversed micelles;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
beta-Xylosidase recovery by reversed micelles using CTAB cationic surfactant was performed under different experimental conditions. A 22-full orthogonal design with center points was employed to optimize the recovery of this enzyme and to evaluate the influence of the factors CTAB ( A) and butanol concentration ( B) on the enzyme extraction. A mathematical model to represent the enzyme recovery as a function of A and B was proposed. According to the model, the CTAB and butanol concentrations necessary to attain the maximum beta-xylosidase recovery (43%) were 0.26 M and 29.4%, respectively. The results showed that the recovery value predicted by the model was similar to that obtained experimentally ( 44.3%).
引用
收藏
页码：153 / 160
页数：8
相关论文
共 18 条
[1]   Xylanase and β-xylosidase separation by fractional precipitation [J].
Cortez, EV ;
Pessoa, A .
PROCESS BIOCHEMISTRY, 1999, 35 (3-4) :277-283
[2]   Screening of variables in β-xylosidase recovery using cetyl trimethyl ammonium bromide reversed micelles [J].
Hasmann, FA ;
Pessoa, A ;
Roberto, IC .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2001, 91-3 (1-9) :719-728
[3]   Optimization of pH and temperature for β-xylosidase recovery by reversed micelles [J].
Hasmann, FA ;
Pessoa, A ;
Roberto, IC .
BIOTECHNOLOGY TECHNIQUES, 1999, 13 (04) :239-242
[4]   β-xylosidase recovery by reversed micelles use of cationic surfactant [J].
Hasmann, FA ;
Pessoa, A ;
Roberto, IC .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2000, 84-6 (1-9) :1101-1111
[5]  
IHARA T, 1995, CHEM PHARM BULL, V43, P626
[6]   Liquid-liquid extraction by reversed micelles in biotechnological processes [J].
Kilikian, BV ;
Bastazin, MR ;
Minami, NM ;
Gonçalves, EMR ;
Pessoa, A .
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2000, 17 (01) :29-38
[7]  
KREI GA, 1992, CHEM ENG SCI, V47
[8]  
Kumar S, 1996, FEMS MICROBIOL LETT, V135, P287, DOI 10.1016/0378-1097(95)00468-8
[9]   REVERSE MICELLES AS HOSTS FOR PROTEINS AND SMALL MOLECULES [J].
LUISI, PL ;
GIOMINI, M ;
PILENI, MP ;
ROBINSON, BH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 947 (01) :209-246
[10]   EXTRACTION OF LYSOZYME AND RIBONUCLEASE-A USING REVERSE MICELLES - LIMITS TO PROTEIN SOLUBILIZATION [J].
LYE, GJ ;
ASENJO, JA ;
PYLE, DL .
BIOTECHNOLOGY AND BIOENGINEERING, 1995, 47 (05) :509-519
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