Renovascular hypertension: Effects of mesenchymal stem cells in the contralateral hypertensive kidney in rats

被引：14

作者：

de Oliveira-Sales, Elizabeth Barbosa ^{[1
]}

Varela, Vanessa Araujo ^{[1
]}

Maquigussa, Edgar ^{[1
]}

Borges, Fernanda Teixeira ^{[1
]}

Shimoura, Caroline Gusson ^{[2
]}

Gomes, Guiomar ^{[2
]}

Campos, Ruy Ribeiro ^{[2
]}

Boim, Mirian Aparecida ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Div Renal, Dept Med, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, Dept Physiol, Sao Paulo, Brazil

来源：

CLINICAL AND EXPERIMENTAL HYPERTENSION | 2016年 / 38卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

Mesenchymal stem cells; renovascular hypertension; renal function; renin-angiotensin system; RENAL-ARTERY STENOSIS; ENDOTHELIAL PROGENITOR CELLS; GOLDBLATT HYPERTENSION; OXIDATIVE STRESS; BLOOD-PRESSURE; MECHANISMS; ISCHEMIA; 2-KIDNEY; INJURY; REVASCULARIZATION;

D O I：

10.3109/10641963.2016.1174253

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Mesenchymal stem cells (MSC) induced neovascularization and improved renal morphology of the stenotic kidney in 2 kidneys-1 clip (2K-1C) model of renovascular hypertension. The present study evaluated the effects of MSC in the contralateral hypertensive kidney. Three weeks after left renal artery occlusion, MSC were injected into the tail vein of the 2K-1C rats. Renal function and morphology were analyzed in both kidneys. Labeled MSC were found in stenotic and contralateral kidneys. Hypertensive 2K-1C animals presented increased circulating levels of Angiotensin II (Ang II) and renin. MSC prevented the progressive increase of blood pressure and reduced circulating Ang II and renin levels. Stenotic kidney showed reduced renal plasma flow (RPF) and glomerular filtration rate (GFR), whereas the contralateral kidney had a tendency (p > 0.5) of reduction in GFR in spite of unchanged RPF. MSC treatment caused an improvement in GFR with no effect of on RPF in the stenotic kidney. Contralateral kidney showed increased diuresis and natriuresis that were even higher in MSC-treated animals, indicating that cell treatment improved the capacity of the contralateral kidney to excrete sodium. Contralateral kidney expressed higher levels of inflammatory cytokines (IL-6, TNF-) and signs of fibrosis, which were attenuated by MSC treatment. MSC treatment improved the stenotic kidney function, and it was also beneficial to the contralateral hypertensive kidney because it improved the morphology and preserved its capacity to excrete sodium.

引用

页码：586 / 593

页数：8

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