Antioxidant and anti-inflammatory polyphenols and peptides of common bean (Phaseolus vulga L.) milk and yogurt in Caco-2 and HT-29 cell models

被引：74

作者：

Chen, Yuhuan ^{[1
,2
]}

Zhang, Hua ^{[2
]}

Liu, Ronghua ^{[2
]}

Mats, Lili ^{[2
]}

Zhu, Honghui ^{[2
]}

Pauls, K. Peter ^{[3
]}

Deng, Zeyuan ^{[1
]}

Tsao, Rong ^{[2
]}

机构：

[1] Univ Nanchang, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

[2] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada

[3] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada

来源：

JOURNAL OF FUNCTIONAL FOODS | 2019年 / 53卷

关键词：

Common bean milk; Common bean yogurt; Peptide; Phenolic; Cellular antioxidant activity; Anti-inflammatory activity; AMINO-ACIDS; PROTEIN; FERMENTATION; INFLAMMATION; PHENOLICS; ANTHOCYANIN; FRACTIONS; CYTOKINES; PROFILES; PINTO;

D O I：

10.1016/j.jff.2018.12.013

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Common bean milks and their corresponding probiotic yogurts were developed and subjected to simulated gastrointestinal digestion before characterization and assessment of bioactivities. Only digestates fractions of molecular weight < 10 kDa showed strong cellular antioxidant activity and anti-inflammatory activity as demonstrated in suppressed TNF-alpha-induced IL-8 secretion in Caco-2 and HT-29 cell lines. Phenolic and peptide fractions were purified from the digestates. Yogurts showed significantly stronger cellular antioxidant and anti-inflammatory activities compared to their corresponding milks because of the significantly higher total and individual phenolic and peptide contents (P < 0.05). Eleven phenolic compounds and five amino acids/peptides were identified in the phenolic and peptide fractions, respectively using LC-MS/MS. Among them, two gamma-glutamyl-dipeptides are reported for the first time for their antioxidant and anti-inflammatory activities. Peptide fractions of yogurts containing higher concentrations of gamma-glutamyl-peptides showed stronger anti-inflammatory activity in Caco-2 cells than in HT-29 cells, suggesting the involvement of a peptide transporter PepT1.

引用

页码：125 / 135

页数：11

共 49 条

[31]

Ohsu T., 2009, J BIOL CHEM JBC M, V109

[32] Common bean (Phaseolus vulgaris L.) hydrolysates inhibit inflammation in LPS-induced macrophages through suppression of NF-κB pathways [J].

Oseguera-Toledo, Miguel E. ;

Gonzalez de Mejia, Elvira ;

Dia, Vermont P. ;

Amaya-Llano, Silvia L. .

FOOD CHEMISTRY, 2011, 127 (03) :1175-1185

[33] A review on protein-phenolic interactions and associated changes [J].

Ozdal, Tugba ;

Capanoglu, Esra ;

Altay, Filiz .

FOOD RESEARCH INTERNATIONAL, 2013, 51 (02) :954-970

[34] The gut microbiota shapes intestinal immune responses during health and disease [J].

Round, June L. ;

Mazmanian, Sarkis K. .

NATURE REVIEWS IMMUNOLOGY, 2009, 9 (05) :313-323

[35] Production of bioactive peptides during soybean fermentation and their potential health benefits [J].

Sanjukta, Samurailatpam ;

Rai, Amit Kumar .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2016, 50 :1-10

[36] Antioxidative peptides from food proteins: A review [J].

Sarmadi, Bahareh H. ;

Ismail, Amin .

PEPTIDES, 2010, 31 (10) :1949-1956

[37]

Shahidi F., 2003, PHENOLICS FOOD NUTRA

[38] Diversity of γ- glutamyl peptides and oligosaccharides, the "kokumi" taste enhancers, in seeds from soybean mini core collections [J].

Shibata, Masayuki ;

Hirotsuka, Motohiko ;

Mizutani, Yukiko ;

Takahashi, Haruya ;

Kawada, Teruo ;

Matsumiya, Kentaro ;

Hayashi, Yukako ;

Matsumura, Yasuki .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2018, 82 (03) :507-514

[39] NUTRITIONAL EVALUATION OF SOY YOGHURT IN COMPARISON TO SOYMILK AND COMMERCIAL MILK YOGHURT. EFFECT OF FERMENTATION ON SOY PROTEIN [J].

Silva Junior, S. I. ;

Tavano, O. L. ;

Demonte, A. ;

Rossi, E. A. ;

Pinto, A. .

ACTA ALIMENTARIA, 2012, 41 (04) :443-450

[40] Effect of controlled lactic acid fermentation on selected bioactive and nutritional parameters of tempeh obtained from unhulled common bean (Phaseolus vulgaris) seeds [J].

Starzynska-Janiszewska, Anna ;

Stodolak, Bozena ;

Mickowska, Barbara .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2014, 94 (02) :359-366

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