Antioxidant and anti-inflammatory polyphenols and peptides of common bean (Phaseolus vulga L.) milk and yogurt in Caco-2 and HT-29 cell models

被引：74

作者：

Chen, Yuhuan ^{[1
,2
]}

Zhang, Hua ^{[2
]}

Liu, Ronghua ^{[2
]}

Mats, Lili ^{[2
]}

Zhu, Honghui ^{[2
]}

Pauls, K. Peter ^{[3
]}

Deng, Zeyuan ^{[1
]}

Tsao, Rong ^{[2
]}

机构：

[1] Univ Nanchang, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

[2] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada

[3] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada

来源：

JOURNAL OF FUNCTIONAL FOODS | 2019年 / 53卷

关键词：

Common bean milk; Common bean yogurt; Peptide; Phenolic; Cellular antioxidant activity; Anti-inflammatory activity; AMINO-ACIDS; PROTEIN; FERMENTATION; INFLAMMATION; PHENOLICS; ANTHOCYANIN; FRACTIONS; CYTOKINES; PROFILES; PINTO;

D O I：

10.1016/j.jff.2018.12.013

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Common bean milks and their corresponding probiotic yogurts were developed and subjected to simulated gastrointestinal digestion before characterization and assessment of bioactivities. Only digestates fractions of molecular weight < 10 kDa showed strong cellular antioxidant activity and anti-inflammatory activity as demonstrated in suppressed TNF-alpha-induced IL-8 secretion in Caco-2 and HT-29 cell lines. Phenolic and peptide fractions were purified from the digestates. Yogurts showed significantly stronger cellular antioxidant and anti-inflammatory activities compared to their corresponding milks because of the significantly higher total and individual phenolic and peptide contents (P < 0.05). Eleven phenolic compounds and five amino acids/peptides were identified in the phenolic and peptide fractions, respectively using LC-MS/MS. Among them, two gamma-glutamyl-dipeptides are reported for the first time for their antioxidant and anti-inflammatory activities. Peptide fractions of yogurts containing higher concentrations of gamma-glutamyl-peptides showed stronger anti-inflammatory activity in Caco-2 cells than in HT-29 cells, suggesting the involvement of a peptide transporter PepT1.

引用

页码：125 / 135

页数：11

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