Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9

被引：3

作者：

Yuan, Fang ^{[1
,2
,3
,4
]}

Guo, Dongsheng ^{[2
,3
,4
,5
]}

Gaob, Ge ^{[2
,3
,4
]}

Liu, Yanli ^{[2
,3
,4
,5
]}

Xu, Yingying ^{[2
,3
,4
,5
]}

Wu, Yuhang ^{[2
,3
,4
]}

Yang, Fan ^{[2
,3
,4
]}

Ke, Xinrong ^{[2
,3
,4
,5
]}

Lai, Keyu ^{[3
]}

Hong, Liangqing ^{[6
]}

Li, Yin-xiong ^{[1
,2
,3
,4
,5
]}

机构：

[1] Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Inst Publ Hlth, Guangzhou, Peoples R China

[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou, Guangdong, Peoples R China

[4] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Biocomputing, Guangzhou, Guangdong, Peoples R China

[5] Univ Chinese Acad Sci, Beijing, Peoples R China

[6] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Kidney Transplantat, Guangzhou, Guangdong, Peoples R China

来源：

STEM CELL RESEARCH | 2017年 / 24卷

关键词：

D O I：

10.1016/j.scr.2017.08.016

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The ATP-sensitive potassium channel is an octameric complex, and one of its subunits, namely Kir6.2, is encoded by the KCNJ11 gene. Mutations in KCNJ11 result in hyperinsulinism or diabetes mellitus, associated with abnormal insulin secretion. Here, using CRISPR/Cas9 editing, we established a homozygous mutant KCNJ11 cell line, WAe001-A12, which was generated by a 62-bp deletion in the coding sequence of the human embryonic stem cell line H1. It was confirmed that this deletion in the KCNJ11 gene did not affect the protein expression levels of key pluripotent factors. Additionally, normal karyotype and differentiation potency were observed for the cell line. (C) 2017 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license

引用

页码：89 / 93

页数：5

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