Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland

被引:11
作者
Higashi, Atsuko Y. [1 ,2 ]
Higashi, Tomohito [1 ]
Furuse, Kyoko [3 ]
Ozeki, Kana [1 ]
Furuse, Mikio [3 ,4 ]
Chiba, Hideki [1 ]
机构
[1] Fukushima Med Univ, Dept Basic Pathol, 1 Hikariga Oka, Fukushima 9601295, Japan
[2] Fukushima Med Univ, Dept Nephrol & Hypertens, Fukushima 9601295, Japan
[3] Natl Inst Physiol Sci, Div Cell Struct, Okazaki, Aichi 4448787, Japan
[4] SOKENDAI Grad Univ Adv Studies, Sch Life Sci, Dept Physiol Sci, Okazaki, Aichi 4448585, Japan
关键词
PROTEIN; OCCLUDIN; ADENOHYPOPHYSIS; CORTICOTROPHS; LOCALIZATION; PERSPECTIVES; EXPRESSION; NETWORK;
D O I
10.1038/s41598-021-01004-z
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, which are sealed by tight junctions (TJs). Although FS cells are hypothesized to contribute to fine-tuning of endocrine cells, little is known about the exact roles of FS cells. Here, we investigated the molecular composition of TJs in FS cells. We demonstrated that occludin is a good marker for TJs in the pituitary gland and examined the structure of the lumens surrounded by FS cells. We also found that claudin-9 is a major component of TJs in the FS cells. In immunoelectron microscopy, claudin-9 was specifically localized at TJs of the FS cells. The expression of claudin-9 was gradually increased in the pituitary gland after birth, suggesting that claudin-9 is developmentally regulated and performs some specific functions on the paracellular barrier of follicles in the pituitary gland. Furthermore, we found that angulin-1, angulin-2, and tricellulin are localized at the tricellular contacts of the FS cells. Our findings provide a first comprehensive molecular profile of TJs in the FS cells, and may lead us towards unveiling the FS cell functions.
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页数:14
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